Identification and High-level Production of Pulcherrimin in Bacillus licheniformis DW2

Applied Biochemistry and Biotechnology
Xiaoyun LiS Chen

Abstract

Pulcherrimin, a potential biocontrol agent produced by microorganisms, has the promising applications in the agricultural, medical, and food areas, and the low yield of pulcherrimin has hindered its applications. In this study, the red pigment produced by Bacillus licheniformis DW2 was identified as pulcherrimin through the spectrometry analysis and genetic manipulation, and the component of the medium used for pulcherrimin production was optimized. Based on our results, the addition of 1.0 g L-1 Tween 80 could improve the yield of pulcherrimin, and glucose and (NH4)2SO4 were served as the optimal carbon and nitrogen sources for pulcherrimin synthesis, respectively. Furthermore, an orthogonal array design was applied for optimization of the medium. Under optimized condition, the maximum yield of pulcherrimin was 331.17 mg L-1, 5.30-fold higher than that of the initial condition, which was the maximum yield reported for pulcherrimin production. Collectively, this study provided a promising strain and a feasible approach to achieve the high-level production of antimicrobial pulcherrimin.

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Citations

Jan 14, 2018·Applied and Environmental Microbiology·Kaifeng LiShouwen Chen
Mar 1, 2020·Applied and Environmental Microbiology·Shiyi WangShouwen Chen
Nov 21, 2019·Applied Microbiology and Biotechnology·Yangyang ZhanShouwen Chen
Oct 21, 2020·Microbial Biotechnology·Satish Kumar RajasekharanMoshe Shemesh
Feb 16, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kęstutis MažeikaVytautas Melvydas

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