Identification and immunohistochemical characterization of colospinal afferent neurons in the rat.

Neuroscience
Shelby K Suckow, Robert M Caudle

Abstract

The classification, morphology and function of enteric neurons have been extensively studied in the small and large intestine. However, little is known about enteric neurons that directly project to the CNS. Previous studies have identified these unique neurons in the rectum, rectospinal neurons, but little was done to characterize them. Therefore, the aim of this study was to identify and characterize enteric neurons in the rat colon that directly project to the CNS by using retrograde neuronal tracing and immunohistochemistry. By applying the retrograde tracers 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) and Fluorogold (FG) to the L6/S1 segments of the spinal cord, we identified these neurons in both the myenteric and submucosal plexuses of the colon. These neurons were immunoreactive for neurofilament (NF) a marker for Adelta-fibers and isolectin-B4 (IB(4)) a marker for C-fibers. These neurons expressed the enzyme neuronal nitric oxide synthase (nNOS) as well as peptides associated with sensory neurons such as substance P (SP) and vasoactive intestinal polypeptide (VIP) but did not express calcitonin gene-related peptide (CGRP). The N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2D and ...Continue Reading

References

Apr 1, 1979·The Journal of Comparative Neurology·K ChungR E Coggeshall
Oct 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J StreitS S Spicer
Apr 1, 1981·Journal of the Autonomic Nervous System·W C de GroatK Thor
Jul 29, 1982·Brain Research·R H LaMotte, J G Thalhammer
Aug 11, 1998·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M Miampamba, K A Sharkey
May 13, 1999·The Journal of Comparative Neurology·C W MorganD E Scott
Nov 18, 2000·Cell and Tissue Research·A E Lomax, J B Furness
Apr 16, 2002·The Journal of Comparative Neurology·Juan Carlos G MarvizónEmeran A Mayer
Apr 26, 2002·Pain·Elie D Al-Chaer, Richard J Traub
Jun 22, 2002·Gut·J H SzurszewskiS M Miller
Aug 20, 2003·The Journal of Comparative Neurology·Sean M WardHans R Berthoud
Apr 6, 2004·Progress in Neurobiology·John B FurnessNadine Clerc
May 29, 2004·Neuroreport·Charles H HubscherRichard D Johnson
Sep 28, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A M HarringtonB R Southwell
Feb 16, 2006·Autonomic Neuroscience : Basic & Clinical·John B Furness
Jun 27, 2006·Autonomic Neuroscience : Basic & Clinical·John B Furness
Nov 18, 2006·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·I DemedtsJ Tack

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Citations

Oct 6, 2011·Seminars in Immunopathology·Andrea S Rothmeier, Wolfram Ruf
Feb 27, 2013·Nature Reviews. Gastroenterology & Hepatology·Simon J H BrookesVladimir P Zagorodnyuk
Sep 26, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pablo Rodolfo BrumovskyG F Gebhart
Nov 13, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bin Feng, G F Gebhart
Aug 15, 2009·Autonomic Neuroscience : Basic & Clinical·P R Brumovsky, G F Gebhart
Dec 16, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S K SuckowR M Caudle
Nov 8, 2014·The Journal of Comparative Neurology·Bao Nan ChenSimon J H Brookes
May 2, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·G CiprianiG Farrugia
Mar 14, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Roeland BuckinxJean-Pierre Timmermans

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