PMID: 2509684Nov 1, 1989Paper

Identification and induction of cytochromes P450, P450IIE1 and P450IA1 in rabbit bone marrow

The Journal of Pharmacology and Experimental Therapeutics
G G SchnierD R Koop

Abstract

A number of drugs and xenobiotics which require metabolic activation to reactive metabolites by cytochrome P450 are toxic to hemopoietic tissue. In the present study we used immunoblot analysis and specific catalytic activities to identify two isozymes of cytochrome P450 (P450IIE1 and P450IA1) in rabbit bone marrow microsomal preparations. P450IIB1, P450IIC1, P450IIIA6 and P450IA2 were below detectable levels using immunoblot analysis. P450IIE1 was present at a concentration of 0.0065 nmol/mg of microsomal protein in marrow microsomes from untreated rabbits, and it was induced 12.9-fold by acetone treatment. The change in the P450IIE1 apoprotein determined immunochemically in bone marrow microsomes after acetone treatment was paralleled by a 9.4-fold increase in benzene hydroxylation, a P450IIE1-dependent activity. Aroclor 1254 pretreatment induced P450IA1 in marrow microsomes 11-fold to a specific content of about 0.025 nmol/mg of microsomal protein. 7-Ethoxyresorufin deethylation, a P450IA1-dependent activity, was induced 15.7-fold by Aroclor 1254 treatment. The increases in the enzymic activities are consistent with an increase in catalytically competent holoenzymes. The presence of these two isozymes in bone marrow could re...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.