PMID: 6159482Nov 1, 1980Paper

Identification and initial characterization of a new low-molecular-weight virus-encoded T antigen in a line of simian virus 40-transformed cells

Journal of Virology
G J SpanglerD M Livingston

Abstract

SV80 cells, a simian virus 40 (SV40)-transformed derivative of a strain of human fibroblasts, synthesize an 8-kilodalton anti-T reactive polypeptide in addition to large T and small t antigens. Although not observed during lytic infection carried out under a variety of conditions, an anti-T reactive molecule which comigrated with the SV80 8-kilodalton protein during sodium dodecyl sulfate-polyacrylamide gel electrophoresis was synthesized by one of five other SV40-transformed cell lines studied. The SV40 8-kilodalton protein was present in lysates of cells exposed to a brief pulse of radioactive methionine and did not accumulate during an extended chase period. This polypeptide could not by generated by mixing an unlabeled extract of SV80 cells with a labeled extract of infected monkey cells. The 8-kilodalton molecule reacts with antibody raised against homogeneous large T antigen, is present only in the cytoplasm, is not complexed with T, lacks DNA-binding properties, and is not phosphorylated. This protein could be translated in a cell-free system programmed by SV40-specific mRNA. At least two messenger species (approximately 19S and approximately 22S) directed its synthesis. Tryptic peptide analysis of [35S]methionine-labele...Continue Reading

Citations

Jan 1, 1982·CRC Critical Reviews in Biochemistry·B Schaffhausen
Sep 1, 1986·Journal of Virology·C I MurphyD M Livingston
Dec 1, 1982·Journal of Virology·M LovettF Chaudry
Dec 1, 1982·Journal of Virology·C E ClaytonP W Rigby
Aug 1, 1997·Journal of Virology·M I RileyW R Folk

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