Identification and isolation of slow-dividing cells in human glioblastoma using carboxy fluorescein succinimidyl ester (CFSE).

Journal of Visualized Experiments : JoVE
Loic P DeleyrolleHassan Azari

Abstract

Tumor heterogeneity represents a fundamental feature supporting tumor robustness and presents a central obstacle to the development of therapeutic strategies(1). To overcome the issue of tumor heterogeneity, it is essential to develop assays and tools enabling phenotypic, (epi)genetic and functional identification and characterization of tumor subpopulations that drive specific disease pathologies and represent clinically relevant targets. It is now well established that tumors exhibit distinct sub-fractions of cells with different frequencies of cell division, and that the functional criteria of being slow cycling is positively associated with tumor formation ability in several cancers including those of the brain, breast, skin and pancreas as well as leukemia(2-8). The fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) has been used for tracking the division frequency of cells in vitro and in vivo in blood-borne tumors and solid tumors such as glioblastoma(2,7,8). The cell-permeant non-fluorescent pro-drug of CFSE is converted by intracellular esterases into a fluorescent compound, which is retained within cells by covalently binding to proteins through reaction of its succinimidyl moiety with intracellular amine g...Continue Reading

Citations

Feb 12, 2019·Journal of Cellular Physiology·Maryam AkbarzadehNasser Samadi
Apr 3, 2013·Current Protocols in Cytometry·A Bruce LyonsKathleen V Doherty
Mar 18, 2020·JCI Insight·Alexander J ColeRonald J Buckanovich
Feb 8, 2020·Cell Death & Disease·André L S CruzPatricia T Bozza

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