Identification and kinetic analysis of a functional homolog of elongation factor 3, YEF3 in Saccharomyces cerevisiae

Yeast
A V SarthyR C Goldman

Abstract

Yeast and other fungi contain a soluble elongation factor 3 (EF-3) which is required for growth and protein synthesis. EF-3 contains two ABC cassettes, and binds and hydrolyses ATP. We identified a homolog of the YEF3 gene in the Saccharomyces cerevisiae genome database. This gene, designated YEF3B, is 84% identical in protein sequence to YEF3, which we will now refer to as YEF3A. YEF3B is not expressed during growth under laboratory conditions, and thus cannot rescue growth of YEF3A deletion strains. However, YEF3B can take the place of YEF3A in vivo when expressed from the YEF3A or ADH1 promoters. The products of the YEF3A and YEF3B genes, EF-3A and EF-3B, respectively, were expressed from the ADH1 promoter and purified. Both factors possessed basal and ribosomal-stimulated ATPase activity, and had similar affinity for yeast ribosomes (103 to 113 nM). K(m) values for ATP were similar, but the Kcat values differed significantly. Ribosome-dependent ATPase activity of EF-3A was more efficient than EF-3B, since the Kcat and Kcat/K(m) values for EF-3A were about two-fold higher; however, the difference in Kcat/K(m) values between the two factors was small for basal ATPase activity.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Aug 27, 1990·Biochimica Et Biophysica Acta·M SandbakenK Chakraburtty
Jan 1, 1991·Methods in Enzymology·I Herskowitz, R E Jensen
Jan 25, 1989·Nucleic Acids Research·M R Slater, E A Craig
Jul 1, 1988·Journal of Biochemistry·M Uritani, M Miyazaki
Jan 1, 1985·Current Genetics·R J LeerR J Planta
Nov 26, 1984·Nucleic Acids Research·J L TeemW H Mager
Sep 1, 1995·The Journal of Biological Chemistry·F J Triana-AlonsoK H Nierhaus
Oct 1, 1994·European Journal of Biochemistry·M V RodninaA V El'Skaya
Aug 1, 1993·Molecular Microbiology·G P Belfield, M F Tuite
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B A HowardC Prescott
Mar 7, 1997·The Journal of Biological Chemistry·R Kambampati, K Chakraburtty

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Citations

Oct 22, 2002·Expert Opinion on Therapeutic Targets·Joy Sturtevant
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shinya KurataHideko Kaji
May 18, 2016·Genetics·Thomas E DeverGraham D Pavitt
Dec 4, 2013·Cellular & Molecular Biology Letters·Agata Piecuch, Ewa Obłąk
Dec 21, 2002·The Journal of Biological Chemistry·Monika AnandTerri Goss Kinzy
Dec 29, 2015·BMC Evolutionary Biology·Andriy KovalchukFred O Asiegbu
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·K Chakraburtty
Mar 6, 1999·Current Opinion in Microbiology·N H Georgopapadakou

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