Identification and localization of acid-base transporters in the conjunctival epithelium

Experimental Eye Research
Helen C TurnerOscar A Candia

Abstract

Acid-base transporters of rabbit and porcine conjunctival epithelia were identified and localized with immunoblotting and immunohistochemical techniques using specific antibodies against carriers commonly found in epithelia, i.e. the Cl(-)/HCO3(-)exchanger (AE2), Na(+)/H(+) exchanger (NHE-1, -2, -3) and the electrogenic Na(+)-(n)HCO3(-) cotransporter (NBC). Western blot analysis demonstrated that anti-AE2 reacted with an approximate 170 kDa protein in both rabbit and pig cell membranes prepared from separately isolated bulbar and palpebral conjunctivae. NHE1 was similarly identified in these distinct conjunctival regions but results with anti-NBC were ambiguous. Histochemical examinations indicated that the AE2 and NHE1 proteins reside on the basolateral surfaces of the plasma membrane throughout the multilayered tissue. The immunostaining of porcine cryosections for AE2 and rabbit sections for NHE1 was specific, because of its abolishment following either pre-absorption with the corresponding peptide or omission of the primary antibody. Screening with anti-NBC produced weak staining of the sections that appeared to be non-specific. For confirmation of these results, the acid-base transporters present in rabbit cell cultures of...Continue Reading

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Citations

Apr 30, 2003·Expert Opinion on Biological Therapy·Surajit DeyAshim K Mitra
Apr 25, 2007·Current Eye Research·Heng Zhu, Anuj Chauhan
Mar 4, 2006·Biochemical and Biophysical Research Communications·Na-Youn KooKyungpyo Park
Apr 27, 2004·Experimental Eye Research·Oscar A Candia
Feb 11, 2005·American Journal of Physiology. Cell Physiology·Lawrence J AlvarezOscar A Candia
Mar 15, 2006·American Journal of Physiology. Cell Physiology·Oscar A CandiaAldo C Zamudio
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Nov 8, 2014·American Journal of Physiology. Cell Physiology·Hua XuFayez K Ghishan

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