PMID: 10980420Sep 12, 2000Paper

Identification and localization of lysozyme as a component of eggshell membranes and eggshell matrix

Matrix Biology : Journal of the International Society for Matrix Biology
M T HinckeY Nys

Abstract

The avian eggshell is a composite biomaterial composed of non-calcifying eggshell membranes and the overlying calcified shell matrix. The calcified shell forms in a uterine fluid where the concentration of different protein species varies between the initial, rapid calcification and terminal phases of eggshell deposition. The role of these avian eggshell matrix proteins during shell formation is poorly understood. The properties of the individual components must be determined in order to gain insight into their function during eggshell mineralization. In this study, we have identified lysozyme as a component of the uterine fluid by microsequencing, and used western blotting, immunofluorescence and colloidal-gold immunocytochemistry to document its localization in the eggshell membranes and the shell matrix. Furthermore, Northern blotting and RT-PCR indicates that there is a gradient to the expression of lysozyme message by different regions of the oviduct, with significant albeit low levels expressed in the isthmus and uterus. Lysozyme protein is abundant in the limiting membrane that circumscribes the egg white and forms the innermost layer of the shell membranes. It is also present in the shell membranes, and in the matrix of...Continue Reading

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Related Concepts

Vaterite
Polymorphism, Crystallization
Egg Shell
Immunocytochemistry
Scanning Electron Microscopy
Leftose
Poly(A) Tail

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