Identification and localization of the nicotinic acetylcholine receptor alpha3 mRNA in the brain of the honeybee, Apis mellifera

Insect Molecular Biology
Steeve Hervé ThanyM Gauthier

Abstract

The nicotinic acetylcholine receptors are ligand-gated ion channels responsible for rapid neurotransmission and are target sites for pesticides in insects. In the honeybee Apis mellifera, pharmacological and electrophysiological studies have shown that different nicotinic acetylcholine receptor subtypes may exist in the brain. Here, we have identified a honeybee cDNA that encodes a 537 amino acid protein with features typical of nicotinic acetylcholine receptor alpha subunit, and sequence homology to human alpha3. In situ hybridization on cryosections shows that the Apisalpha3 mRNA is differently expressed in larvae and adult. In larvae, Apisalpha3 mRNA expression is restricted to the suboesophageal ganglia. In adult, it is further expressed in the optic lobes, the dorsal lobes, the antennal lobes and the calyces of mushroom bodies. Together our results suggest that Apisalpha3 shows a controlled expression pattern during development.

References

Jun 1, 1992·Trends in Neurosciences·E D Gundelfinger
Nov 17, 1992·Biochimica Et Biophysica Acta·E D Gundelfinger, N Hess
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·D Benke, H Breer
May 10, 1995·Annals of the New York Academy of Sciences·J LindstromY Li
Aug 1, 1996·European Journal of Biochemistry·F HuchoJ Machold
Dec 24, 1997·Brain Research. Brain Research Reviews·H R Arias
May 20, 1998·Pharmacology, Biochemistry, and Behavior·V Cano Lozano, M Gauthier
Jul 11, 1998·The Journal of Biological Chemistry·B HermsenA Maelicke
Sep 30, 1998·The European Journal of Neuroscience·H M EasthamS Wonnacott
Apr 20, 2000·European Journal of Pharmacology·L M Marubio, J Changeux
Jun 6, 2000·Trends in Pharmacological Sciences·M Cordero-ErausquinJ P Changeux
Jul 27, 2001·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·V C LozanoM Gauthier
Mar 2, 2002·Neuroscience Letters·Patrice DégliseMonique Gauthier

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Citations

Aug 17, 2004·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Daniel G Wüstenberg, Bernd Grünewald
Aug 12, 2006·Invertebrate Neuroscience : in·Andrew K Jones, David B Sattelle
Jan 12, 2007·Invertebrate Neuroscience : in·Neil S Millar, Ian Denholm
Nov 16, 2007·Invertebrate Neuroscience : in·Guillaume Stéphane BarbaraValérie Raymond-Delpech
Apr 28, 2004·Insect Biochemistry and Molecular Biology·Adriana Mendes do NascimentoKlaus Hartfelder
Oct 27, 2006·Genome Research·Andrew K JonesDavid B Sattelle
Mar 22, 2014·PloS One·Johannes FischerRandolf Menzel
Jul 5, 2008·Physiology & Behavior·M Dacher, M Gauthier
Sep 17, 2014·Environmental Science and Pollution Research International·L W PisaM Wiemers
May 28, 2020·Frontiers in Physiology·Alison CartereauSteeve H Thany

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