Identification and molecular characterisation of a fibrinogen binding protein from Streptococcus iniae.

BMC Microbiology
J BaianoAndrew C Barnes

Abstract

Binding of serum components by surface M-related proteins, encoded by the emm genes, in streptococci constitutes a major virulence factor in this important group of organisms. The present study demonstrates fibrinogen binding by S. iniae, a Lancefield non-typeable pathogen causing devastating fish losses in the aquaculture industry and an opportunistic pathogen of humans, and identifies the proteins involved and their encoding genes. Fibrinogen binding by S. iniae significantly reduced respiratory burst activity of barramundi peritoneal macrophages in primary cultures compared to BSA-treated or untreated controls, indicating a potentially important role for fibrinogen binding cell-surface proteins in avoiding phagocytic attack in fish. We describe a novel emm-like gene, simA, encoding a 57 kDa fibrinogen binding M-like protein in S. iniae. These SiM proteins and their corresponding tetrameric structures from some sequevar types (approximately 230 kDa) bound fibrinogen in Western blots. simA was most closely related (32% identity) to the demA gene of S. dysgalactiae. Genome walking and sequencing determined the genetic organization of the simA region had similarities to the mgrC regulon in GCS and to S. uberis. Moreover, a putat...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Jan 1, 1996·Methods in Enzymology·A Lupas
Aug 28, 1997·The New England Journal of Medicine·M R WeinsteinD E Low
Dec 22, 1999·Infection and Immunity·J VasiB Guss
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin
Dec 4, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Richard F FacklamBernard Beall
Mar 16, 2002·Infection and Immunity·Mary C HuJames B Dale
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·James C SmootJames M Musser
Apr 15, 2003·Diseases of Aquatic Organisms·Andrew C BarnesAnthony E Ellis
Oct 3, 2003·Microbial Pathogenesis·Igor MoshynskyyAndrew A Potter
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nilay DeyHarpreet Vohra
Feb 8, 2005·Journal of Clinical Microbiology·Richard FacklamArt Reingold
Sep 24, 2005·Infection and Immunity·John T BuchananVictor Nizet
Jan 20, 2006·Molecular Microbiology·Harry S CourtneyJames B Dale
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephen B BeresJames M Musser
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ellen Hsu, Michael F Criscitiello
Oct 3, 2006·Journal of Bacteriology·Matthew T G HoldenJulian Parkhill
Nov 14, 2006·Journal of Bacteriology·Jeffrey B LockeJohn T Buchanan

❮ Previous
Next ❯

Citations

Mar 10, 2009·Applied and Environmental Microbiology·Roslina A NawawiAndrew C Barnes
Oct 24, 2012·Infection and Immunity·Elizabeth A HarvieAnna Huttenlocher
Dec 8, 2009·Emerging Infectious Diseases·Justice C F Baiano, Andrew C Barnes
Jun 24, 2014·Microbes and Infection·Fatima El AamriDaniel Padilla

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
dot-blot
PCR
fluorescence
lavage
electrophoresis

Software Mentioned

COIL

Related Concepts

Related Feeds

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.