Identification and molecular characterization of Serratia marcescens phages vB_SmaA_2050H1 and vB_SmaM_2050HW

Archives of Virology
Changyu TianXiangna Zhao

Abstract

Serratia marcescens is a rod-shaped, Gram-negative bacterium causing nosocomially acquired infections. Bacteriophages are natural opponents of their pathogenic bacterial hosts and could be an alternative to traditional antibiotic treatments. In this study, two S. marcescens-specific bacteriophages, vB_SmaA_2050H1 and vB_SmaM_2050HW, were isolated from two different waste samples in China. Phage plaque assays, transmission electron microscopy, host-range determination, and one-step growth curve analyses were performed for both phages. vB_SmaA_2050H1 was classified as belonging to the family Ackermannviridae, and vB_SmaM_2050HW was classified as belonging to the family Myoviridae. One-step growth curve analysis showed that the latent and rise period of vB_SmaA_2050H1 were 80 min and 50 min, respectively, with a burst size of approximately 103 phage particles per infected cell. For vB_SmaM_2050HW, latent and rise periods of 40 min and 60 min, respectively, were determined, with a burst size of approximately 110 phage particles per infected cell. vB_SmaA_2050H1 infected 10 of the 15 (66.67%) S. marcescens strains tested, while vB_SmaM_2050HW infected 12 (80%) of the strains. Whole-genome sequencing and annotation of each of the pha...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 15, 1959·Experientia·R FISCHER, F GRIFFIN
Jan 6, 2004·Nucleic Acids Research·Dean Laslett, Bjorn Canback
Jul 3, 2004·Genome Research·Aaron C E DarlingNicole T Perna
Apr 1, 1983·Applied and Environmental Microbiology·J J Germida, L E Casida
Dec 11, 2008·Methods in Molecular Biology·Hans-W Ackermann
Dec 11, 2008·Methods in Molecular Biology·Hans-W Ackermann
Dec 11, 2008·Methods in Molecular Biology·Elizabeth Kutter
May 21, 2011·Virology Journal·Hany AnanyAndrew M Kropinski
Sep 9, 2011·Virology Journal·Elizabeth M KutterAndrew D Brabban
Aug 28, 2012·Journal of Virology·Sung-Hun KimShukho Kim
Nov 21, 2012·Journal of Virology·Miguel A Matilla, George P C Salmond
Jan 18, 2014·Virology Journal·Jenna M DenyesAndrew M Kropinski
Apr 4, 2017·Viruses·Evelien Adriaenssens, J Rodney Brister
Jun 10, 2017·Genome Announcements·Eoghan CaseyDouwe van Sinderen
Jan 6, 2018·Genome Announcements·Yuchong HaoYanbo Sun

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Citations

Sep 17, 2020·International Journal of Molecular Sciences·Katarzyna BujakMonika Radlinska
Jul 20, 2019·Microbiology Resource Announcements·Lyndsey PriceJolene Ramsey
Aug 5, 2021·Annual Review of Microbiology·Tyler B JacobsonDaniel Amador-Noguez

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Datasets Mentioned

BETA
MF285619
MF285618

Methods Mentioned

BETA
PCR

Software Mentioned

TMHMM
CLC Main Workbench
ClustalW
Basic Local Alignment Search Tool ( BLAST )
Mauve
MEGA

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