Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance.

The Journal of Biological Chemistry
J KieboomG J Zylstra

Abstract

Bacteria able to grow in aqueous:organic two-phase systems have evolved resistance mechanisms to the toxic effects of solvents. One such mechanism is the active efflux of solvents from the cell, preserving the integrity of the cell interior. Pseudomonas putida S12 is resistant to a wide variety of normally detrimental solvents due to the action of such an efflux pump. The genes for this solvent efflux pump were cloned from P. putida S12 and their nucleotide sequence determined. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins known to be involved in proton-dependent multidrug efflux systems. Transfer of the genes for the solvent efflux pump to solvent-sensitive P. putida strains results in the acquisition of solvent resistance. This opens up the possibilities of using the solvent efflux system to construct bacterial strains capable of performing biocatalytic transformations of insoluble substrates in two-phase aqueous:organic medium.

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, D Gillespie
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1990·Applied and Environmental Microbiology·S HartmansJ A de Bont
May 1, 1966·Journal of General Microbiology·R Y StanierM Doudoroff
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Jun 1, 1995·Microbiological Reviews·J SikkemaB Poolman
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·A A FerranteA M Klibanov
Jul 1, 1995·Bioscience, Biotechnology, and Biochemistry·H NakajimaR Aono
Dec 1, 1994·Applied and Environmental Microbiology·H J Heipieper, J A de Bont
Oct 1, 1993·Applied and Environmental Microbiology·F J WeberJ A de Bont
Dec 1, 1995·Bioscience, Biotechnology, and Biochemistry·L LiK Horikoshi
Oct 1, 1996·Journal of Bacteriology·H Nikaido
Oct 1, 1996·Journal of Bacteriology·S Isken, J A de Bont
Feb 1, 1996·Lipids·H Keweloh, H J Heipieper
Oct 29, 1996·Biochimica Et Biophysica Acta·F J Weber, J A de Bont
Dec 1, 1996·Microbiological Reviews·I T PaulsenR A Skurray
Feb 14, 1997·The Journal of Biological Chemistry·J L RamosA Fernández-Barrero
Aug 1, 1996·Applied and Environmental Microbiology·H J HeipieperJ de Bont

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Citations

Oct 16, 2002·Biotechnology and Bioengineering·In Seon KimMurray R Gray
Mar 12, 2011·Die Naturwissenschaften·Francesco Canganella, Juergen Wiegel
Mar 1, 2012·Applied Microbiology and Biotechnology·Abigail A AdebusuyiJulia M Foght
Oct 21, 2005·Extremophiles : Life Under Extreme Conditions·Zileena ZahirJonathan J Dennis
Jun 8, 2006·Journal of Industrial Microbiology & Biotechnology·Balsam T MohammadMark T Bustard
Aug 20, 2011·Journal of Industrial Microbiology & Biotechnology·N K HarnerJ T Trevors
Sep 2, 2010·Systems and Synthetic Biology·Mary J DunlopAindrila Mukhopadhyay
Jun 9, 2005·Advanced Drug Delivery Reviews·Ayush Kumar, Herbert P Schweizer
Mar 17, 2004·FEMS Microbiology Letters·Florence JudeClaudine Quentin
Sep 19, 2003·International Journal of Antimicrobial Agents·Pedro FernandesJoaquim Manuel Sampaio Cabral
Jun 14, 2000·Current Opinion in Biotechnology·D T Gibson, R E Parales
Oct 20, 2005·Journal of Bioscience and Bioengineering·Michiaki MatsumotoSonja Isken
Feb 24, 2001·Environmental Microbiology·A SeguraF Junker
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·K TsugeM Shoda
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Xian-Zhi LiKeith Poole
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Wilson TeránMaría-Trinidad Gallegos
Oct 31, 2000·Applied and Environmental Microbiology·D R KadavyK W Nickerson
Aug 30, 2001·Applied and Environmental Microbiology·M I Ramos-GonzálezJ L Ramos
Dec 8, 2005·Applied and Environmental Microbiology·Nick J P WierckxJan Wery
Jul 21, 2006·Journal of Bacteriology·Jasper Kieboom, Tjakko Abee
Dec 31, 2008·Journal of Bacteriology·Sandy FilletJuan L Ramos
Oct 29, 2000·Journal of Bacteriology·H KobayashiK Horikoshi

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