Identification and molecular characterization of a 27 kDa Shigella flexneri invasion plasmid antigen, IpaJ

Microbial Pathogenesis
J M BuysseM M Venkatesan

Abstract

Shigella species and enteroinvasive Escherichia coli contain a core set of virulence genes whose coordinated expression results in the invasion of host colonic epithelial cells and the dysenteric syndrome. A number of virulence determinants are carried by the 230 kb invasion plasmid found in all virulent strains of Shigellae. Many of these invasion plasmid genes encode immunogens that are recognized by convalescent serum, including proteins that mediate the invasion (IpaB, IpaC, IpaD) and cell spreading (VirG or IcsA and IcsB) phenotypes. In this report, we describe the molecular characterization of a novel invasion plasmid antigen from Shigella flexneri, designated IpaJ. The ipaJ gene encodes a 780 bp open reading frame (ORF), separated from the ipaR (virB) stop codon by 944 bp. The predicted amino acid sequence for IpaJ revealed a consensus signal peptide for protein export. TnphoA mutagenesis of the ipaJ ORF confirmed the presence of export signal sequences in IpaJ. Unlike ipaBCDA genes, transcription analysis of ipaJ indicated that the gene is not expressed in a temperature-dependent fashion. The IpaJ protein was expressed and purified as a His6-tagged fusion protein that reacted with convalescent sera in Western blot analy...Continue Reading

References

Aug 7, 1992·Cell·C S Shean, M E Gottesman
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Mar 25, 1990·Nucleic Acids Research·M M Venkatesan, J M Buysse
May 1, 1990·The Journal of Membrane Biology·G von Heijne
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BernardiniP J Sansonetti
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Manoil, J Beckwith
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M M VenkatesanD J Kopecko
Apr 1, 1985·Infection and Immunity·E V OaksS B Formal
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Oct 1, 1981·Journal of Bacteriology·S GottesmanP Trisler
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K ItoY Nakamura
Sep 1, 1994·Molecular Microbiology·J W Izard, D A Kendall
Feb 1, 1994·The Journal of Clinical Investigation·J J PerdomoP J Sansonetti
Jan 1, 1994·Methods in Enzymology·E A Elsinghorst
Jan 1, 1994·Methods in Enzymology·D J Kopecko
Mar 1, 1993·Microbiological Reviews·A P Pugsley

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Citations

Apr 11, 2017·Frontiers in Cellular and Infection Microbiology·Emily Mattock, Ariel J Blocker
Sep 27, 2018·MBio·Benjamin J KoestlerShelley M Payne
Oct 24, 2020·Trends in Microbiology·Ligang MeiAimin Yang
Dec 1, 2004·EcoSal Plus·Charles J Dorman

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