Identification and molecular typing of Naegleria fowleri from a patient with primary amebic meningoencephalitis in China

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Ling-ling ZhangLi-nong Yao

Abstract

Naegleria fowleri is the only Naegleria spp. known to cause an acute, fulminant, and rapidly fatal central nervous system infection in humans called primary amebic meningoencephalitis (PAM). In 2016, a patient with suspected PAM was found in Zhejiang Province of China. The pathogen was identified by microscopic examination and PCR. The positive PCR products were sequenced and the sequences were aligned using the NCBI BLAST program. The homologous and phylogenetic analysis was conducted using MEGA 6 program. On microscopy of direct smears, motile cells with pseudopodia were observed, and the motion characteristics of the pseudopodia as well as the cell morphology suggested that the pathogens were amoeba trophozoites. Wright-Giemsa-stained smears showed amoeba trophozoites of various shapes, which measured 10-25μm in size; these were characterized by a prominent, centrally placed nucleolus and a vacuolated cytoplasm. PCR was negative for Entamoeba histolytica and Entamoeba dispar, but positive for Naegleria spp. and N. fowleri. The nucleotide sequences acquired in this study have been submitted to GenBank with accession numbers KX909928 and KX909927, respectively. The BLAST analysis revealed that the sequences of KX909928 and KX9...Continue Reading

Citations

Oct 16, 2019·Clinical and Experimental Pharmacology & Physiology·Muhammad JahangeerRida Zainab
Nov 18, 2019·Parasitology Research·Hossain M S SazzadIbne K M Ali
Apr 13, 2021·Parasite : Journal De La Société Française De Parasitologie·Libia Zulema Rodriguez-AnayaJose Reyes Gonzalez-Galaviz

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