Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
R ChakrabortyD van der Helm

Abstract

Many gram-negative bacteria produce and excrete siderophores, which complex iron with high affinity in the environment. The ferric siderophore complexes are transported across the outer membrane by receptor proteins. This process requires energy and is TonB dependent and must involve conformational changes in the receptor proteins to allow the transport of the ferric siderophores from the extracellular binding site to the periplasm. There is a large variety in the structures, molecular weights and charges among the siderophores. It was therefore realized that when the sequences of the many different receptor proteins were compared, simultaneously, all identities and close similarities, found in this manner, could only be due to residues involved in the conformational changes and transport mechanism, common to all the proteins, and not be due to the specificity of ligand recognition. Once the crystal structures of FepA, FhuA and FecA became available, it was immediately clear that the sequence similarities which were found in the simultaneous alignment, were all localized in a few structural domains, which are identical in the three structures and can therefore be expected to be maintained in all the proteins in this family. One...Continue Reading

Citations

Nov 2, 2004·Journal of Bacteriology·Krisztina M Papp, Michael E Maguire
Jun 8, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·E P StoreyR Chakraborty
Dec 8, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Milva PepiRoberto Bargagli
Mar 8, 2017·Journal of Bacteriology·Michael G Gresock, Kathleen Postle
Feb 7, 2020·Molecular & Cellular Proteomics : MCP·Quentin PerraudIsabelle J Schalk
Dec 23, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ranjan ChakrabortyDick van der Helm
Feb 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Claudia S López, Jorge H Crosa
Dec 19, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Gabriel Soares Guerra, Andrea Balan
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Sep 12, 2018·Scientific Reports·Jérémy EsqueChristophe Oguey
Sep 29, 2011·Infection and Immunity·Aimee HollanderCynthia Nau Cornelissen
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Quentin PerraudIsabelle J Schalk
Jul 7, 2021·International Journal of Peptide Research and Therapeutics·Fatemeh Nemati ZargaranMosayeb Rostamian
Jul 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Hamish G UpritchardA James McQuillan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
R ChakrabortyD van der Helm
Frontiers in Bioscience : a Journal and Virtual Library
P E Klebba
International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Seyed Mehdi Larrie BaghalIraj Rasooli
© 2022 Meta ULC. All rights reserved