Identification and organization of genes for diutan polysaccharide synthesis from Sphingomonas sp. ATCC 53159.

Journal of Industrial Microbiology & Biotechnology
Russell J ColemanN E Harding

Abstract

A cluster of genes for diutan polysaccharide synthesis was isolated from a library of Sphingomonas sp. ATCC 53159 genomic DNA by complementation of glucosyl-isoprenylphosphate transferase-deficient mutants of Sphingomonas elodea ATCC 31461 (producing gellan) and Xanthomonas campestris (producing xanthan). The synthesis of polysaccharide in these strains shares a common first step, transfer of glucose-1-phosphate from UDP-glucose to the isoprenylphosphate lipid. The cluster of 24 genes was compared to genes for biosynthesis of gellan, and S-88 sphingan from Sphingomonas sp. ATCC 31554. Diutan, gellan and S-88 sphingan have a common four-sugar backbone but different side chains, one rhamnose for S-88 sphingan, a two-rhamnose side chain for diutan and no side chain for gellan. The genes for biosynthesis of diutan, gellan and S-88 sphingan were similar in general organization but differed in location of some genes, in particular, dpsG (putative polymerase), dpsR (putative lyase) and dpsS (putative repeat unit transporter). An unidentified reading frame urf31, present in the gene clusters for diutan and S-88 sphingan but not gellan, had similarity to glycosyl transferase group 2 proteins, and was detrimental when cloned in Sphingomo...Continue Reading

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Citations

May 29, 2008·Applied Microbiology and Biotechnology·Arsenio M FialhoIsabel Sá-Correia
Aug 2, 2014·Applied Microbiology and Biotechnology·Jochen SchmidVolker Sieber
Dec 24, 2015·Frontiers in Microbiology·Flemming H Larsen, Søren B Engelsen
May 13, 2011·Trends in Biotechnology·Filomena FreitasMaria A M Reis
Jun 16, 2015·Frontiers in Microbiology·Jochen SchmidBernd Rehm
Oct 24, 2014·Frontiers in Chemistry·Christine Delbarre-LadratSylvia Colliec-Jouault
Apr 16, 2015·Chembiochem : a European Journal of Chemical Biology·Jochen Schmid, Volker Sieber
Jan 19, 2018·Applied Microbiology and Biotechnology·Dipanjan SenguptaDipa Biswas
Jan 14, 2011·Journal of Industrial Microbiology & Biotechnology·Liang ZhuHaichun Gao
Feb 3, 2021·Journal of Microbiology and Biotechnology·Sang-Hyeok ChoSuhyung Cho

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