PMID: 2103573Oct 1, 1990Paper

Identification and partial characterization of parathyroid hormone-related protein in human and bovine milk

The Journal of Endocrinology
W A RatcliffeJ G Ratcliffe

Abstract

Parathyroid hormone-related protein (PTHrP) was measured in human and bovine milk by radioimmunoassay (RIA) and bioassay, and the molecular forms characterized by gel chromatography and immunoblotting of affinity-purified PTHrP. Mean immunoreactive PTHrP(1-34) concentrations were 23 and 87 micrograms/l in human and bovine milk respectively. Bioactive (BIO) PTHrP concentrations determined by cyclic AMP production by ROS 17/2.8 cells correlated significantly (P less than 0.001) with those obtained by RIA (BIO = 1.04RIA--3.4, r = 0.939). Gel filtration of human and bovine milk identified several peaks with immunoactivity and bioactivity. Immunoblotting of affinity-purified PTHrP revealed multiple molecular species including components with mobilities similar to those of PTHrP and its subfragments. These studies confirm the presence of immuno- and bioactive PTHrP in milk and suggest that post-translational processing is complex and variable.

Citations

Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·F LawJ P Bonjour
Jan 13, 1994·Biochimica Et Biophysica Acta·J F EmlyW A Ratcliffe
Aug 1, 1994·Comparative Biochemistry and Physiology. Comparative Physiology·G E ThompsonA D Care
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R C EdwardsN J Bundred
Sep 24, 2009·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Ronit LubetzkyDror Mandel
Nov 5, 2003·The Journal of Clinical Investigation·Joshua N VanHoutenJohn J Wysolmerski
Jan 1, 1995·Critical Reviews in Clinical Laboratory Sciences·J M Moseley, M T Gillespie
Aug 8, 2006·The Journal of Veterinary Medical Science·Ken OndaYasunori Wada
Nov 1, 1992·Clinical Endocrinology·W A Ratcliffe
Oct 1, 1995·Clinical Otolaryngology and Allied Sciences·I M CheshireD W Proops
Sep 1, 1994·Acta Paediatrica. Supplement·O Koldovský
Mar 1, 1993·Clinical Endocrinology·S J BowdenW A Ratcliffe
Feb 8, 2006·The Journal of Veterinary Medical Science·Ken OndaYasunori Wada
Jun 15, 2006·American Journal of Physiology. Endocrinology and Metabolism·Deenaz ZaidiGraham F Wagner
Dec 1, 1992·The American Journal of Physiology·M RakopoulosT J Martin
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