PMID: 6990002Mar 31, 1980Paper

Identification and partial characterization of five major membrane glycoproteins of BHK fibroblasts

The Journal of Membrane Biology
G TaroneP M Comoglio

Abstract

Five major membrane glycoproteins of the BHK-B4 hamster fibroblast plasma membrane have been identified by binding specific rabbit antibodies to the cell surface and by recovering the detergent solubilized immunocomplexes with Protein A-Sepharose immunoadsorption. These glycoproteins, designated as gp45, gp65, gp95, gp130 and gp140, are exposed at the cell surface since: (i) they were accessible to antibodies in intact viable cells; (ii) they were radioiodinated by the lactoperoxidase-glucose oxidase procedure; and (iii) they were cleaved by proteolytic enzymes in conditions affecting only the cell surface. Among these glycoproteins the gp130 is the predominant component and its exposed portion is characterized by lack of sensitivity to trypsin cleavage. Glycoproteins of different molecular weight, but immunologically related to the major hamster membrane glycoproteins, have been detected at the surface of both rat and mouse fibroblasts.

References

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Citations

Aug 1, 1986·The Journal of Cell Biology·F G GiancottiG Tarone
Mar 15, 1988·European Journal of Biochemistry·G TaroneF G Giancotti
Jun 15, 1981·International Journal of Cancer. Journal International Du Cancer·M PratP M Comoglio
Apr 15, 1982·International Journal of Cancer. Journal International Du Cancer·R FerraciniP M Comoglio

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