PMID: 9439004Jan 24, 1998Paper

Identification and partial characterization of tochicin, a bacteriocin offduced by Bacillus thuringiensis subsp tochigiensis

Journal of Industrial Microbiology & Biotechnology
H D PaikJ G Pan

Abstract

Bacillus thuringiensis subsp tochigiensis HD868 was identified as a bacteriocin producer which exhibited a bactericidal effect against closely related species. This bacteriocin designated as tochicin, was partially purified by 75% ammonium sulfate precipitation followed by subsequent dialysis. This partially purified tochicin showed a narrow antibacterial spectrum of activity against most of 20 typical B. thuringiensis strains and a strain of B. cereus, but not against other bacteria and yeasts tested. The antibacterial activity of tochicin on sensitive indicator cells disappeared completely by proteinase K treatment (1 mg ml-1), which indicates its proteinaceous nature. Tochicin was very stable throughout the range of pH 3.0-9.0 and was relatively heat-stable at 90 degrees C, but bacteriocin activity was not detected after boiling for 30 min. The relationship between cell growth and bacteriocin production was studied in a semi-defined medium. Tochicin activity was detected at the mid-log growth phase, reached the maximum at the early stationary phase, but decreased after the stationary phase. Direct detection of tochicin activity on sodium dodecyl sulfate-polyacrylamide gel suggested it has an apparent molecular mass of about ...Continue Reading

Citations

Oct 13, 2006·Archives of Microbiology·J Eleazar Barboza-CoronaRubén Salcedo-Hernández
Apr 30, 2011·Biotechnology Letters·Estibaliz Sansinenea, Aurelio Ortiz
Sep 1, 2009·Indian Journal of Microbiology·Neha Gautam, Nivedita Sharma
Mar 20, 2003·FEMS Microbiology Letters·Melissa AhernDouwe van Sinderen
Mar 20, 2010·Canadian Journal of Microbiology·Sonia ChehimiFérid Limam
Jan 14, 2011·Lipids in Health and Disease·Aida KarraySofiane Bezzine
Jul 26, 2008·Archives of Microbiology·N de la Fuente-SalcidoJ E Barboza-Corona
Jun 10, 2004·Journal of Applied Microbiology·N RaddadiD Daffonchio
Jun 25, 2004·Letters in Applied Microbiology·F Cladera-OliveraA Brandelli
Mar 12, 2004·Journal of Applied Microbiology·P A RisøenA-B Kolstø
Dec 12, 2012·Journal of Applied Microbiology·S Smitha, S G Bhat
Jul 13, 2004·International Journal of Food Microbiology·Sungsook BaeGillian M Heard
Aug 11, 2010·FEMS Microbiology Reviews·Hikmate AbriouelAntonio Gálvez
Jan 30, 2015·Canadian Journal of Microbiology·Chandra Datta SumiYoung Tae Hahm
Aug 30, 2018·Mini Reviews in Medicinal Chemistry·Aurelio Ortiz, Estibaliz Sansinenea
Dec 21, 2019·Applied Microbiology and Biotechnology·Chetan KeswaniEstibaliz Sansinenea
Nov 19, 2015·Frontiers in Plant Science·Sowmyalakshmi Subramanian, Donald L Smith
Jun 25, 2016·Reviews in Medical Microbiology : a Journal of the Pathological Society of Great Britain and Ireland·Elma Laura Salazar-MarroquínBenito Pereyra-Alférez

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