Identification and pathogenicity of Vibrio parahaemolyticus isolates and immune responses of Penaeus (Litopenaeus) vannamei (Boone)

Journal of Fish Diseases
X P HongL Q Lu

Abstract

Five different Vibrio parahaemolyticus strains (SH8, SH108, SH58, AH5 and GD10) isolated from the hepatopancreas of moribund shrimp in farms of mainland China were identified and capable of inducing massive mortality of Penaeus (Litopenaeus) vannamei. The immersion challenge results with five isolates indicated variance of virulence, while only GD10 caused massive sloughing of tubule epithelial cells which was recognized as the most significant symptom of AHPND. Differences in immune responses were detected of P. vannamei during 48 h post-infection (p.i.) by injection or immersion challenge with V. parahaemolyticus (SH8, SH108 and GD10) isolates. When injected SH8 and SH108 isolates, the expression of lysozyme (LSZ) showing statistically significant upregulation at 16 and 48 h p.i. and that of Toll-like receptors (TLR) showed statistically significant upregulation at 48 h p.i. When immersion challenge with the GD10 isolate, TLR were upregulated after 8 h p.i. challenge with 10(4)  cfu mL(-1) ; however, LSZ was downregulated when challenged with 10(3)  cfu mL(-1) . The results suggested that LSZ and TLR serve as crucial molecular markers of innate immunity in shrimp against V. parahaemolyticus infection. LSZ is a vital marker fo...Continue Reading

References

May 1, 1985·Journal of Bacteriology·M Nishibuchi, J B Kaper
Dec 31, 2003·Molecular Immunology·Bruce Beutler
Oct 24, 2006·Molecular Immunology·Li-Shi YangJian-Guo He
Dec 16, 2011·Journal of Clinical Microbiology·Allen E TealSusan Madison-Antenucci
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Aug 22, 2014·Diseases of Aquatic Organisms·Linda NunanSilvia Gomez-Jimenez
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chung-Te LeeChu-Fang Lo

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Citations

Nov 30, 2019·Diseases of Aquatic Organisms·Gerardo de Carrillo-MéndezCésar Salvador Cardona-Félix

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