Apr 1, 1986

Identification and physical characterization of yeast glucoamylase structural genes

Molecular & General Genetics : MGG
I S PretoriusJ Marmur

Abstract

Each one of at least three unlinked STA loci (STA1, STA2 and STA3), in the genome of Saccharomyces diastaticus controls starch hydrolysis by coding for an extracellular glucoamylase. Cloned STA2 sequences were used as hybridization probes to investigate the physical structure of the family of STA genes in the genomes of different Saccharomyces strains. Sta+ strains, each carrying a single genetically defined STA locus, were crossed with a Sta- strain and the segregation behavior of the functional locus (i.e. Sta+) and sequences homologous to a cloned STA2 glucoamylase structural gene at that locus were analyzed. The results indicate that in all strains examined there is a multiplicity of sequences that are homologous to STA2 DNA but that only the functional STA loci contain extensive 5' and 3' homology to each other and can be identified as residing on unique fragments of DNA; that all laboratory yeast strains examined contain extensive regions of the glucoamylase gene sequences at or closely linked to the STA1 chromosomal position; that the STA1 locus contains two distinct glucoamylase gene sequences that are closely linked to each other; and that all laboratory strains examined also contain another ubiquitous sequence that is...Continue Reading

  • References11
  • Citations15

References

Mentioned in this Paper

Glucan 1,4-alpha-Glucosidase
Streptomyces diastaticus
Genome
Extracellular
Maltose Metabolic Process
Dextrin
Nucleic Acid Hybridization Procedure
EMD gene
Saccharomyces
Genes, Fungal

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