Identification and preliminary characterization of Hc-clec-160, a novel C-type lectin domain-containing gene of the strongylid nematode Haemonchus contortus

Parasites & Vectors
Ling ZhangAifang Du

Abstract

The strongylid parasite Haemonchus contortus causes severe anemia in domestic animals worldwide. Effective preventive and therapeutical agents are lacking, because of drug resistance and that little is known about the molecular mechanism of the interaction between H. contortus and host cells. A new gene, Hc-clec-160, was discovered with RT-PCR. Transcriptional levels of Hc-clec-160 and Ce-clec-160 throughout different growth phases of corresponding nematodes were assayed by qPCR. Immunofluorescence staining of paraffin section were performed to determine the protein localization in adult worms of H. contortus. To monitor the promoter capacity of the 5'-flanking region of Ce-clec-160, micro-injection was used. Overexpression and RNAi constructs was carried out in the N2 strain of Caenorhabditis elegans to find out the gene function of Hc-clec-160. The full-length cDNA of 1224 bp of Hc-clec-160 was cloned by RT-PCR. The corresponding gene contained twelve exons. Its transcripts peaked in male adult worms. Hc-CLEC-160 was predicted to have a Willebrand factor type A (vWA) domain and a C-type lectin domain. The proteins were not detected by expression in C. elegans or paraffin section experiments in adult of H. contortus. Knockdown...Continue Reading

References

Jan 1, 1987·Advances in Parasitology·J M Behnke
Oct 6, 1999·Current Opinion in Structural Biology·K Drickamer
Feb 1, 2000·Parasitology Today·J Chernin
Jul 20, 2000·Parasitology Today·A Loukas, R M Maizels
Sep 2, 2003·Nature Reviews. Immunology·Rick M Maizels, Maria Yazdanbakhsh
Feb 6, 2004·Glycoconjugate Journal·Anna R Young, Els N Meeusen
Dec 13, 2005·The FEBS Journal·Alex N Zelensky, Jill E Gready
Aug 8, 2006·International Journal for Parasitology·John Stuart Gilleard
Mar 14, 2008·Nature·Ronald KaminskyPascal Mäser
May 16, 2009·Nature Reviews. Microbiology·Gerardo R Vasta
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shaobing O ZhangHo Yi Mak
Mar 6, 2013·PLoS Pathogens·Christina M TaylorMakedonka Mitreva
Mar 26, 2013·Veterinary Parasitology·Enrico Lippi OrtolaniNeville F Suttle
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

❮ Previous
Next ❯

Datasets Mentioned

BETA
AAY58318.1
KKA67531.1
ERG83551.1

Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
fluorescence microscopy
transgenic

Software Mentioned

Clustal W
Excel
MEGA

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.