Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits

Biochemical and Biophysical Research Communications
C TonC C Liew

Abstract

The multi-subunit NADH-ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. A small number of NADH-ubiquinone oxidoreductase subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. We have isolated and sequenced five subunits of the human complex I from a human heart lambda ZAP Express cDNA library. Comparison of the deduced amino acid sequences of the human subunits with the corresponding bovine sequences revealed greater than 80% amino acid identity. The high degree of similarity between human and bovine sequences suggests functional conservation of these subunits in the complex I. In silico Northern analysis revealed that two of the subunits were expressed ubiquitously while the remainder may have more restricted patterns of expression.

Citations

May 21, 2005·Cell Death and Differentiation·J S LadhaD Mitra
Oct 10, 1998·Journal of Paediatrics and Child Health·T F LeungK Goh
Jul 3, 2007·Nucleic Acids Research·Paulien SmitsThijs J G Ettema
Jan 13, 2006·Journal of Ethnopharmacology·Chia-Sheng ChenMing-Tsuen Hsieh
Oct 5, 2010·Biochemical and Biophysical Research Communications·Ruben S R M MartherusTorik A Y Ayoubi
Jan 30, 2016·Scientific Reports·Richard E FryeMichael J Goldenthal
Mar 2, 2012·Journal of Child Neurology·Richard E Frye
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·L L XuS Srivastava
Dec 5, 2002·Physiological Genomics·Amparo C VillablancaJohn C Rutledge
May 11, 1999·Journal of Inherited Metabolic Disease·R TriepelsL van den Heuvel
Jan 8, 1999·Biochemical and Biophysical Research Communications·J L LoeffenJ A Smeitink

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