Identification and profiling of miRNAs in the freeze-avoiding gall moth Epiblema scudderiana via next-generation sequencing

Molecular and Cellular Biochemistry
Pierre J LyonsPier Morin

Abstract

The rapid development of high-throughput next-generation sequencing approaches in recent years has facilitated large-scale discovery and expression analysis of non-coding RNAs, including miRNAs, in traditional and non-traditional animal models. Such an approach has been leveraged to amplify, identify, and quantify miRNAs in several models of cold adaptation. The present study is the first to investigate the status of these small RNAs in an insect species that uses the freeze avoidance strategy of cold hardiness, the gall moth Epiblema scudderiana. To characterize the overall miRNA expression profile and to identify cold-modulated miRNAs in control (5 °C) and cold-exposed (-15 °C) E. scudderiana larvae, a next-generation sequencing-based approach was undertaken. A total of 44 differentially expressed miRNAs were identified between the two conditions; 21 up-regulated miRNAs and 23 down-regulated miRNAs in -15 °C-exposed larvae as compared with controls. Among the most significant changes observed in miRNAs with potential relevance to cold adaptation were elevated miR-1-3p, miR-92b-3p, and miR-133-3p levels as well as reduced miR-13a-3p and miR-13b-3p levels in E. scudderiana larvae exposed to cold temperatures. Expression values ...Continue Reading

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Citations

Oct 17, 2018·The Journal of Experimental Biology·Simon G EnglishKenneth B Storey
Nov 21, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Jacques J FrigaultPier Jr Morin
Apr 24, 2018·Cellular and Molecular Life Sciences : CMLS·Hanane Hadj-Moussa, Kenneth B Storey
Dec 6, 2017·Journal of Molecular Cell Biology·Kyle K Biggar, Kenneth B Storey
Feb 28, 2019·PeerJ·Myriam P HoyeckKenneth B Storey
Mar 17, 2017·Insect Biochemistry and Molecular Biology·Julie A ReynoldsDavid L Denlinger
Aug 20, 2021·Journal of Insect Physiology·Sam M WilliamsonKenneth B Storey

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