Identification and purification of a hemoglobin-binding outer membrane protein from Neisseria gonorrhoeae.

Infection and Immunity
C J ChenC Elkins

Abstract

The majority of in vitro-grown Neisseria gonorrhoeae strains were unable to use hemoglobin as the sole source of iron for growth (Hgb-), but a minor population was able to do so (Hgb+). The ability of Hgb+ gonococci to utilize hemoglobin as the iron source was associated with the expression of an iron-repressible 89-kDa hemoglobin-binding protein in the outer membrane. The N-terminal amino acid sequence of this protein revealed amino acids, from positions 2 to 16, identical to those of HpuB, an 85 kDa iron-regulated hemoglobin-haptoglobin utilization outer membrane protein of Neisseria meningitidis. Isogenic mutants constructed by allelic replacement with a meningococcal hpu::mini-Tn3erm construct no longer expressed the 89-kDa protein. Mutants could not utilize hemoglobin to support growth but still grew on heme. Thus, the gonococcal HpuB homolog is a functional hemoglobin receptor and is essential for growth with hemoglobin.

References

Feb 1, 1977·Journal of Bacteriology·G D BiswasP F Sparling
Mar 1, 1978·Microbiological Reviews·E D Weinberg
Feb 1, 1992·Journal of Medical Microbiology·B C Lee
Dec 1, 1992·Journal of General Microbiology·B C Lee, P Hill
Jan 1, 1992·Critical Reviews in Microbiology·B R OttoD M MacLaren
Oct 1, 1991·Journal of Bacteriology·J M RutzP E Klebba
Apr 1, 1988·Infection and Immunity·W R McKennaD W Dyer
Nov 12, 1970·The New England Journal of Medicine·U Muller-Eberhard
Aug 1, 1982·Infection and Immunity·L W Mayer
Dec 1, 1995·Infection and Immunity·P J DesaiC A Genco
Dec 1, 1994·Molecular Microbiology·C N Cornelissen, P F Sparling
Dec 1, 1995·Journal of Bacteriology·R J DanaherD C Stein
Nov 1, 1995·Molecular Microbiology·B C Lee
Jun 1, 1963·Journal of Bacteriology·D S KELLOGGD I PIRKLE

❮ Previous
Next ❯

Citations

Jan 23, 1999·The Journal of Biological Chemistry·S SenguptaA Mukhopadhyay
Jun 26, 1999·Molecular Microbiology·A B Schryvers, I Stojiljkovic
Mar 20, 2003·Journal of Bacteriology·Ying Du, Cindy Grove Arvidson
Jun 6, 2003·Molecular Microbiology·James E AndersonP Frederick Sparling
Oct 27, 2004·Infection and Immunity·Heather L AlexanderIgor Stojiljkovic
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Jul 26, 2005·Infection and Immunity·Mengyao Liu, Benfang Lei
Dec 7, 2007·Infection and Immunity·Yih-Ling TzengDavid S Stephens
Dec 8, 2004·The Journal of Biological Chemistry·Ganga KrishnamurthyAmitabha Mukhopadhyay
Sep 29, 2011·Infection and Immunity·Aimee HollanderCynthia Nau Cornelissen
Jan 6, 2011·Microbiology and Immunology·Hideharu YukitakeKoji Nakayama
Jul 12, 2002·Canadian Journal of Microbiology·Lisa-Anne Agiato Foster
Oct 27, 2009·Journal of Bacteriology·Lydgia A JacksonDavid W Dyer
Dec 26, 2001·Journal of Bacteriology·Ching-Ju ChenP Frederick Sparling
Apr 23, 2004·Molecular Microbiology·Heather L AlexanderIgor Stojiljkovic
Jul 8, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Xuesong SunQing-Yu He
May 4, 2016·Molecular & Cellular Proteomics : MCP·Ryszard A ZielkeAleksandra E Sikora
Mar 27, 1999·Journal of Bacteriology·A R Richardson, I Stojiljkovic
Oct 19, 2017·Pathogens and Disease·Cynthia Nau Cornelissen
Apr 28, 1999·Journal of Bacteriology·S D CarsonP F Sparling
Nov 4, 2017·Fish & Shellfish Immunology·Hossam AbdelhamedAttila Karsi
Mar 6, 1998·Infection and Immunity·C J ChenP F Sparling
May 25, 1999·Infection and Immunity·S Sebastian, C A Genco
Jan 24, 2002·Journal of the American Society of Nephrology : JASN·Jakub GburekErik I Christensen
Oct 2, 2002·Journal of Clinical Microbiology·Kristine PattersonChristopher Elkins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.