PMID: 9431684Jan 1, 1997Paper

Identification and purification of a spinach chloroplast DNA-binding protein that interacts specifically with the plastid psaA-psaB-rps14 promoter region

Planta
M C ChengS C Chen

Abstract

We have previously shown the presence in chloroplasts of sequence-specific DNA-binding proteins that interact specifically with two regions located downstream and upstream from the 5'-transcription start site of the plastid psaA-psaB-rps14 operon. As part of an effort to elucidate the regulatory mechanism of plastid transcription during plant development, we report here the purification and characterization of the chloroplast DNA-binding protein from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) leaves that specifically recognizes sequences between positions +64 to +83 relative to the transcription start site. This DNA-binding protein has been highly purified from chloroplasts by using a combination of high-salt extraction, ammonium sulfate precipitation, heparin-agarose chromatography, and sequence-specific DNA-affinity chromatography. The protein exhibited an apparent molecular weight of 59-60 kDa on the basis of gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Southwestern blot analysis further indicated that this DNA-binding protein is dimeric and composed of two approximately 31-kDa subunits. We discuss the properties of this protein in relation to the known chloroplast ...Continue Reading

Citations

Apr 5, 2001·Trends in Plant Science·N Sato
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yusuke YagiTakashi Shiina
May 14, 2005·Antioxidants & Redox Signaling·Thomas Pfannschmidt, Karsten Liere
Jul 15, 2006·Nucleic Acids Research·Laura M Patterson-FortinGeorge W Owttrim
Sep 26, 2012·The Plant Journal : for Cell and Molecular Biology·Rodrigo CarocaRalph Bock
Jul 4, 2007·Cellular & Molecular Biology Letters·Wojciech PladerStefan Malepszy
Nov 25, 2004·The Journal of Biological Chemistry·Vidal FeyThomas Pfannschmidt
Feb 16, 2006·Environmental Science & Technology·Sam Cherian, M Margarida Oliveira

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