Identification and purification of resveratrol targeting proteins using immobilized resveratrol affinity chromatography

Biochemical and Biophysical Research Communications
Zhirong WangJoseph M Wu

Abstract

The phytochemical resveratrol (trans-3,4',5-trihydroxystilbene) is a naturally occurring polyphenol with a plethora of health-beneficial properties, including a preventive role in cancer. We surmise that resveratrol may exert its diverse biological effects by interacting with specific target proteins, denoted RTPs. To test this possibility, resveratrol was immobilized on epoxy-activated agarose forming a resveratrol affinity column (RAC), which was used to detect and isolate RTPs. Distinct RTPs can be resolved on RAC by fractionation with increasing NaCl, followed by 1mM ATP, and finally, with 1-2mM resveratrol. A 22-kDa polypeptide, RTP-22, eluted with resveratrol was identified by MALDI-TOF MS and cloning/expression in Escherichia coli, as dihydronicotinamide riboside quinone reductase 2 (NQO2). The utility of RAC was additionally explored with extracts derived from different staging prostate cancer cells. NQO2 was most abundant in CWR22Rv1, a model for prostate cancer transition from androgen-dependent to the hormone-refractory state, but was marginally expressed in JCA-1 cells as representing more advanced stage prostate cancer. These results provide evidence for the existence of distinctive RTPs in mammalian cells and that...Continue Reading

References

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B D GehmJ L Jameson
Feb 19, 1998·FEBS Letters·M FontecaveO Guittet
Feb 19, 2000·Life Sciences·L Frémont
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C R Wolf
Jun 16, 2001·International Journal of Molecular Medicine·J M WuY Z Huang
Jul 3, 2002·Nature Reviews. Cancer·Michael B Sporn, Nanjoo Suh
Aug 1, 2002·Leukemia & Lymphoma·Min-Fu TsanG Chikkappa
Jan 16, 2003·Biochimica Et Biophysica Acta·Simon J SlaterChristopher J Buzas
Aug 27, 2003·Nature·Konrad T HowitzDavid A Sinclair
Aug 28, 2003·Drug News & Perspectives·Vinicio Granados-Soto
Mar 18, 2004·Cancer Research·Srinivasan YegnasubramanianWilliam G Nelson
May 4, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Barry D GehmJ Larry Jameson

❮ Previous
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Citations

Jan 29, 2013·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·A F Alex, N Eter
Jan 26, 2011·Journal of Natural Products·Jian-Hong YangHong-Jie Zhang
Mar 4, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Wei WangAnil K Jaiswal
Aug 2, 2008·Clinical and Experimental Pharmacology & Physiology·Jing-Bo CaiKe-Jiang Cao
Nov 4, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lalita SubramanianPaul R van Ginkel
Sep 14, 2011·Investigational New Drugs·Yoshihiko FujitaKazuto Nishio
Dec 5, 2015·Biochemistry·Kevin Ka Ki Leung, Brian Herbert Shilton
Nov 29, 2011·Fitoterapia·Mudasir A TantryKhalid Ghazanfar
Jul 14, 2010·BioFactors·Tze-chen Hsieh, Joseph M Wu
Jan 26, 2011·Annals of the New York Academy of Sciences·Joseph M Wu, Tze-chen Hsieh
Jul 5, 2005·Biochemical and Biophysical Research Communications·Tze-chen HsiehJoseph M Wu
Oct 29, 2005·Biochemical Pharmacology·Fanny VellaJean A Boutin
Apr 20, 2010·Chemico-biological Interactions·Gilles FerryJean A Boutin
Sep 1, 2005·Biochemical and Biophysical Research Communications·Yue FuZhongtao Zhang
Sep 1, 2015·Annals of the New York Academy of Sciences·Ole Vang
Jun 24, 2015·Annals of the New York Academy of Sciences·Robert G BrittonKaren Brown
Jan 18, 2015·Chemico-biological Interactions·Rudolf AndrýsVladimír Wsól
May 11, 2010·The Biochemical Journal·Barbara CalaminiAndrew D Mesecar
Sep 13, 2018·Journal of Drug Targeting·Cong-Hui ZhangWu-Li Zhao
Apr 19, 2008·IUBMB Life·Luciano Pirola, Sara Fröjdö
Jul 19, 2018·Oncotarget·Danielly C Ferraz da CostaJerson L Silva
Dec 3, 2016·Oncotarget·Alicja SznarkowskaKrzysztof Piotr Bielawski
Apr 8, 2017·Applied Microbiology and Biotechnology·Xueli HouCuiyan Wang
Apr 21, 2011·Chembiochem : a European Journal of Chemical Biology·Marine DufourChristopher J Moody
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paul R van GinkelArthur S Polans
Nov 10, 2020·Heliyon·Miguel Ortiz-FloresGuillermo Ceballos

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