Abstract
Disseminated infection with the dimorphic pathogenic fungus Penicillium marneffei is increasingly seen among patients with AIDS in southeast Asian countries. Previous studies have demonstrated the presence of humoral immune responses to this fungus in patient sera; we have confirmed this work using sera from P. marneffei-infected patients (n = 21) to develop Western blots of P. marneffei cytoplasmic yeast antigen (CYA). P. marneffei CYA was then partially purified by liquid isoelectric focusing, and fractions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Immunoenzyme development of the Western blots with pooled sera from patients with P. marneffei infection and with pooled sera from patients with aspergillosis (n = 20), candidiasis (n = 10), cryptococcosis (n = 9), and histoplasmosis (n = 11) revealed three antigens with relative molecular masses of 61, 54, and 50 kDa. These antigens were specifically recognized by the pooled sera from the P. marneffei-infected patients. The 61- and 54-kDa antigens were subsequently purified to homogeneity by preparative gel electrophoresis, and the 50-kDa antigen was partially purified by the same technique. N-terminal amino acid s...Continue Reading
References
Jan 1, 1992·Infection and Immunity·A J HamiltonR J Hay
Sep 1, 1988·Histopathology·D N TsangN K Chan
Mar 27, 1981·Science·C R MerrilM H Ebert
Sep 1, 1981·Analytical Biochemistry·S M Read, D H Northcote
Aug 1, 1995·Journal of Clinical Microbiology·L KaufmanB L Swisher
Dec 1, 1995·Infection and Immunity·R López-MedranoF Leal
Jul 9, 1994·Lancet·K SupparatpinyoT Sirisanthana
Aug 13, 1994·Lancet·K Y YuenP Y Chau
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·L JeavonsA Hamilton
Jun 1, 1996·The Journal of Infectious Diseases·S ChariyalertsakK E Nelson
Jul 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T A Duong
Mar 1, 1996·Clinical and Diagnostic Laboratory Immunology·B L OrtizJ G McEwen
Mar 1, 1997·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·N VanittanakomT Sirisanthana
Sep 1, 1997·Journal of Clinical Microbiology·P ChongtrakoolS Sirisinha
Nov 30, 1959·Mycopathologia et mycologia applicata·G SEGRETAIN
Citations
Nov 22, 2011·The British Journal of Dermatology·N O'ReillyK Kavanagh
Jul 19, 2013·Medical Mycology·Monsicha PongpomNongnuch Vanittanakom
Nov 24, 1999·Oral Diseases·W Nittayananta
Jan 27, 2000·Medical Mycology·A J Hamilton, M D Holdom
Apr 20, 2002·Current Opinion in Infectious Diseases·Rataporn Ungpakorn
Jan 19, 2006·Clinical Microbiology Reviews·Nongnuch VanittanakomThira Sirisanthana
Apr 5, 2002·Microbiology·Clayton H JohnsonJoan E McEwen
Feb 4, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K PrakitS Youngchim
Jul 9, 2010·Medical Mycology·Monsicha Pongpom, Nongnuch Vanittanakom
Jan 27, 2000·Medical Mycology·S YoungchimA J Hamilton
Apr 12, 2018·PloS One·Kritsada PruksaphonSirida Youngchim
Dec 5, 2020·Scientific Reports·Kritsada PruksaphonSirida Youngchim
Dec 15, 2019·Diagnostic Microbiology and Infectious Disease·Kritsada PruksaphonSirida Youngchim
Aug 20, 2017·BMC Microbiology·Xiaoying DaiZhiyi He
May 1, 2021·Diagnostics·Pakornswit SathongdejwisitSirida Youngchim
May 5, 2021·PLoS Neglected Tropical Diseases·Kritsada PruksaphonSirida Youngchim
Jun 22, 2001·Microbes and Infection·C A da FonsecaC M Soares
Apr 3, 2001·Journal of Clinical Microbiology·S S WongK Y Yuen
Dec 17, 1998·Journal of Clinical Microbiology·V DesakornN J White
May 1, 2002·Journal of Clinical Microbiology·Nongnuch VanittanakomRoderick J Hay
Aug 31, 2002·Journal of Clinical Microbiology·Varunee DesakornNicholas J White