PMID: 9542914May 23, 1998Paper

Identification and purification of specific Penicillium marneffei antigens and their recognition by human immune sera

Journal of Clinical Microbiology
L JeavonsR J Hay

Abstract

Disseminated infection with the dimorphic pathogenic fungus Penicillium marneffei is increasingly seen among patients with AIDS in southeast Asian countries. Previous studies have demonstrated the presence of humoral immune responses to this fungus in patient sera; we have confirmed this work using sera from P. marneffei-infected patients (n = 21) to develop Western blots of P. marneffei cytoplasmic yeast antigen (CYA). P. marneffei CYA was then partially purified by liquid isoelectric focusing, and fractions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Immunoenzyme development of the Western blots with pooled sera from patients with P. marneffei infection and with pooled sera from patients with aspergillosis (n = 20), candidiasis (n = 10), cryptococcosis (n = 9), and histoplasmosis (n = 11) revealed three antigens with relative molecular masses of 61, 54, and 50 kDa. These antigens were specifically recognized by the pooled sera from the P. marneffei-infected patients. The 61- and 54-kDa antigens were subsequently purified to homogeneity by preparative gel electrophoresis, and the 50-kDa antigen was partially purified by the same technique. N-terminal amino acid s...Continue Reading

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Citations

Nov 22, 2011·The British Journal of Dermatology·N O'ReillyK Kavanagh
Jul 19, 2013·Medical Mycology·Monsicha PongpomNongnuch Vanittanakom
Jan 27, 2000·Medical Mycology·A J Hamilton, M D Holdom
Apr 20, 2002·Current Opinion in Infectious Diseases·Rataporn Ungpakorn
Jan 19, 2006·Clinical Microbiology Reviews·Nongnuch VanittanakomThira Sirisanthana
Apr 5, 2002·Microbiology·Clayton H JohnsonJoan E McEwen
Feb 4, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K PrakitS Youngchim
Dec 15, 2019·Diagnostic Microbiology and Infectious Disease·Kritsada PruksaphonSirida Youngchim
May 1, 2002·Journal of Clinical Microbiology·Nongnuch VanittanakomRoderick J Hay
Aug 31, 2002·Journal of Clinical Microbiology·Varunee DesakornNicholas J White

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