Identification and quantification of anthocyanin pigments in colored rice.

Nutrition Research and Practice
Min-Kyoung KimYong Ho Kim

Abstract

Anthocyanin pigments from varieties of black, red and wild rice were identified and quantified to evaluate their potential as nutritional function, natural colorants or functional food ingredients. Anthocyanin extraction was conducted with acidified methanol with 0.1M HCl (85:15, v/v) and identification of anthocyanin, aglycone and sugar moieties was conducted by comparison with purified standards by HPLC, Ultraviolet-Visible absorption spectrophotometer and paper chromatography. Black and wild rice showed three different types of pigments by HPLC whereas red rice variety did not show any anthocyanins. Out of three pigments detected, one (peak 2) was characterized as cyanidin-3-glucoside (C3G) by comparison of spectroscopic and chromatographic properties with an authentic standard, and another (peak 3) was tentatively identified as cyanidin-fructoside on the basis of spectroscopic properties with lambda(max) of aglycone in 1% HCl methanol at 537 nm, electrospray ionization mass spectra with major ions at 449 and 287 m/z and chromatographic properties. But another pigment (peak 1) has not been characterized. The most abundant anthocyanin in black and wild rice was C3G.

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Citations

Feb 5, 2014·Journal of Food Science and Technology·Kwangoh KohHee-Seon Kim
Jan 19, 2016·Food Chemistry·Sakina Hayashi, Emiko Yanase
May 3, 2016·Food Chemistry·Gabriela Hörnke AlvesMaurício de Oliveira
Apr 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pavel A ZykinAnatoly V Voylokov
Jul 3, 2019·Nutrients·Bogdan Nicolae ManolescuIleana C Farcasanu
Feb 24, 2017·Foods·Valentina Melini, Rita Acquistucci
Jan 31, 2019·Combinatorial Chemistry & High Throughput Screening·Yunhua ZhangLijuan Chen
Oct 18, 2019·Journal of Nutritional Science and Vitaminology·Jae-Sung LeeAjay Kohli
Jun 30, 2019·International Journal of Biological Macromolecules·Nuttinee TangsrianugulManop Suphantharika

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