Identification and quantification of domestic feline cytochrome P450 transcriptome across multiple tissues

Journal of Veterinary Pharmacology and Therapeutics
Marike VisserD Merritt

Abstract

Understanding of cytochrome P450 (CYP) isoform distribution and function in the domestic feline is limited. Only a few studies have defined individual CYP isoforms across metabolically relevant tissues, hampering the ability to predict drug metabolism and potential drug-drug interactions. Using RNA sequencing (RNA-seq), transcriptomes from the 99 Lives Cat Genome Sequencing Initiative databank combined with experimentally acquired whole transcriptome sequencing of healthy, adult male (n = 2) and female (n = 2) domestic felines, expression of 42 CYP isoforms were identified in 20 different tissues. Thirty-seven of these isoforms had not been previously reported in cats. Depending on the tissue, three to twenty-nine CYP isoform transcripts were expressed. The feline genome annotations did not differentiate CYP2E1 and 2E2 genes, demonstrating poor annotation for this gene using the reference genome. As the majority of the sequences are based on automated pipelines, complete cDNA sequences for translation into CYP protein sequences could not be determined. This study is the first to identify and characterize 37 additional CYP isoforms in feline tissues, increasing the number of identified CYP from the previously reported seven isof...Continue Reading

References

Apr 20, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J J BogaardsB Walther
Mar 15, 2003·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M J GrahamM S Lennard
Apr 23, 2003·Trends in Pharmacological Sciences·Sean EkinsJ Andrew Williams
May 5, 2004·Genome Research·Val CurwenMichele Clamp
Sep 20, 2005·Biochimica Et Biophysica Acta·Nagako TanakaHiroshi Yokota
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Sep 7, 2007·Journal of Veterinary Pharmacology and Therapeutics·S S ShahM Shimoda
Nov 16, 2007·Pharmacology & Therapeutics·Magnus Ingelman-SundbergCristina Rodriguez-Antona
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Samuel Marguerat, Jürg Bähler
Dec 3, 2009·Human Genomics·David R Nelson
Jul 28, 2010·The Journal of Veterinary Medical Science·Tetsuya KomatsuHiroki Teraoka
Jun 30, 2011·The Journal of Veterinary Medical Science·Kouichi HondaHiroki Teraoka
Aug 17, 2011·Genome Research·Lewis Z HongGregory S Barsh
Jan 10, 2012·Science China. Life Sciences·Geng ChenTieliu Shi
Jan 31, 2013·Journal of Clinical Pharmacology·Evan D Kharasch, Kristi Stubbert
Feb 26, 2013·Drug Metabolism Reviews·Marilyn N MartinezLauren Trepanier
Apr 19, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Haruka NishimutaMasashi Yabuki
Apr 26, 2013·Theory in Biosciences = Theorie in Den Biowissenschaften·Günter P WagnerVincent J Lynch
Jul 31, 2013·The Veterinary Clinics of North America. Small Animal Practice·Michael H Court
Oct 30, 2014·Nucleic Acids Research·Fiona CunninghamPaul Flicek
Dec 31, 2014·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Gaku OkamatsuHiroki Teraoka
Feb 14, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Veronika BachanovaJatinder Kaur Lamba
Mar 10, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Gaku OkamatsuHiroki Teraoka
May 18, 2016·The Journal of Pharmacology and Experimental Therapeutics·Hai-Feng ZhangHai-Ling Qiao
Jun 9, 2016·Trends in Pharmacological Sciences·F Peter GuengerichMartin Egli
Oct 23, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Danielle AberdeinUNKNOWN 99 Lives Consortium
Nov 15, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Gaku OkamatsuHiroki Teraoka
Feb 25, 2017·Journal of Veterinary Internal Medicine·D A MaulerUNKNOWN and 99 Lives Consortium

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.