Identification and quantification of the osmodiuretic mannitol in urine for sports drug testing using gas chromatography-mass spectrometry

European Journal of Mass Spectrometry
Sven GuddatW Schänzer

Abstract

The osmodiuretic mannitol can be potentially misused in sports, owing to its urine diluting effect and the possibility to decrease bodyweight. To reveal a doping offence, resulting urinary mannitol concentrations after a prohibited intravenous application and a permitted oral intake have to be differentiated. Therefore, a reliable gas chromatography-mass spectrometry (GC-MS) method was established based on peracetyl derivatives of the analytes. All possible hexitols (allitol, galactitol, iditol, altritols, sorbitol and mannitol) that can occur in human urine were separated and identified on a phenyl-methylpolysiloxane column (HP-5MS) within 10.75 min, and the method demonstrated its capability for quantification purposes. The lower limit of detection and lower limit of quantification were estimated at 0.9 microg mL(-1) and 2.4 microg mL(-1), respectively, and the assay was validated for mannitol and sorbitol regarding the parameters specificity, linearity, intra- (<10%) and inter-day precision (<15%) and accuracy (92-102%). To investigate urinary mannitol concentrations after oral intake the method was applied to an excretion study, providing a mean urinary excretion of mannitol of 19.5%. Comparison of theoretically expected ur...Continue Reading

References

Jul 1, 1980·Biomedical Mass Spectrometry·B R PettitK Blau
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Citations

Mar 23, 2011·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Jul 20, 2010·Annual Review of Analytical Chemistry·Larry D Bowers
Jan 22, 2011·Bioanalysis·Ruth E SimoniFrancisco R Aquino Neto
Apr 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying DongJinglin Ding
Nov 7, 2015·Biomedical Chromatography : BMC·Homayon Ahmad PanahiMonireh Chabouk
Apr 18, 2020·Critical Reviews in Biotechnology·Gheorghe Adrian MartăuDan Cristian Vodnar
Dec 15, 2018·Drug Testing and Analysis·Tim Sobolevsky, Brian Ahrens

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