Identification and quantitation of cis-ketoconazole impurity by capillary zone electrophoresis-mass spectrometry

Journal of Chromatography. a
María Castro-PuyanaMaria Luisa Marina

Abstract

trans-Ketoconazole was identified and quantified as impurity of cis-ketoconazole, an antifungal compound, by capillary zone electrophoresis-electrospray-mass spectrometry (CZE-ESI-MS). The chirality of this impurity was demonstrated separating their enantiomers by adding heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin to the separation buffer in capillary electrophoresis (CE) with UV detection. However, MS detection was hyphenated to the CE instrument for its identification. As both compounds are diastereomers, they have the same m/z values and are needed to be separated prior to the MS identification. A 0.4M ammonium formate separation buffer at pH 3.0 enabled the separation of the impurity from cis-ketoconazole. Under these conditions, the optimization of ESI-MS parameters (composition and flow of the sheath-liquid, drying temperature, drying gas flow, and capillary potential) was carried out to obtain the best MS sensitivity. CZE-ESI-MS optimized conditions enabled the identification of trans-ketoconazole as impurity of cis-ketoconazole. In addition, the quantitation of this impurity was achieved in different samples: cis-ketoconazole standard and three different pharmaceutical formulations (two tablets and one syrup) contai...Continue Reading

References

Dec 9, 2003·Electrophoresis·Philippe Schmitt-Kopplin, Moritz Frommberger
Dec 9, 2003·Electrophoresis·Chun-Che LinShu-Hui Chen
Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes

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Citations

Jul 8, 2014·PloS One·Aneta NovotnaZdenek Dvorak
Apr 24, 2012·Journal of Pharmaceutical and Biomedical Analysis·Saranjit SinghRavi P Shah
Mar 27, 2007·Journal of Pharmaceutical and Biomedical Analysis·J A Bertinatto RodríguezL E Diaz
Sep 27, 2008·Electrophoresis·Abolghasem Jouyban, Ernst Kenndler
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin
Mar 5, 2011·Journal of Separation Science·Ana Valéria Colnaghi SimionatoMarina Franco Maggi Tavares
Jan 3, 2007·Journal of Mass Spectrometry : JMS
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.