PMID: 3773515Sep 1, 1986Paper

Identification and quantitation of free and conjugated steroids in milk from lactating women

Journal of Steroid Biochemistry
B L Sahlberg, M Axelson

Abstract

A method for analysis of metabolic profiles of free and conjugated steroids in milk has been developed. Milk is diluted with aqueous triethylamine sulphate and liquid-solid extraction is achieved on a Sep-Pak C18 cartridge at 60-64 degrees C. Steroids are purified by chromatography on small columns of Lipidex 5000 and sulphohydroxypropyl Sephadex LH-20 [H+] prior to separation into neutral and phenolic compounds, glucuronide, mono- and disulphate conjugate groups on the lipophilic strong anion exchanger triethylaminohydroxypropyl Sephadex LH-20 (TEAP-LH-20). Conjugated steroids are released by enzymatic or solvolytic procedures and separated into a neutral and a phenolic fraction on TEAP-LH-20. The O-methyloxime and trimethylsilyl ether derivatives of the steroids are analyzed by capillary column gas chromatography-mass spectrometry. Fifty steroids were identified in milk collected from women a few days after delivery. Quantitatively about 80% were present as sulphates, 15% as glucuronides and only 5% were unconjugated steroids. The steroid pattern was similar to that in late pregnancy plasma with pregnanolone, pregnanediol and pregnanetriol isomers and dehydroepiandrosterone being predominant. About 10% of the steroid content ...Continue Reading

References

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Citations

Apr 6, 2006·Cancer Causes & Control : CCC·Maddalena BarbaJo L Freudenheim
Feb 18, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bibian van der VoornAnnemieke C Heijboer
Nov 5, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Karl John KarnakyDavid S Miller
Aug 2, 2013·American Journal of Human Biology : the Official Journal of the Human Biology Council·Amanda L Thompson, Michelle Lampl
Sep 24, 2009·Molecular Nutrition & Food Research·Alicja MortensenGerhard Sontag
Mar 17, 1989·Journal of Chromatography·J UusijärviJ Sjövall
Jan 5, 2002·Journal of Chromatography. a·O Nozaki
Dec 10, 2017·Best Practice & Research. Clinical Endocrinology & Metabolism·Jonneke J HollandersMartijn J J Finken

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