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Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses

Letters in Applied Microbiology

Jan 1, 2019

Lisa F StinsonMatthew S Payne

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Abstract

Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to...read more

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Alkaline DNase
Attention
Biomass
Decontamination
DNA
DNA, Bacterial
Indicators
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Treatment Protocols
182
1
55
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Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses

Letters in Applied Microbiology

Jan 1, 2019

Lisa F StinsonMatthew S Payne

PMID: 30383890

DOI: 10.1111/lam.13091

Abstract

Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to...read more

Mentioned in this Paper

Alkaline DNase
Attention
Biomass
Decontamination
DNA
182
1
55

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