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Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses

Letters in Applied Microbiology

Jan 1, 2019

Lisa F StinsonMatthew S Payne

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Abstract

Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to...read more

Mentioned in this Paper

Polymerase Chain Reaction
Treatment Protocols
RNA, Ribosomal, 16S
Study
Extraction
Genome Sequencing
DNA, Bacterial
Sequence Determinations, DNA
DNA Contamination
Bacterial 16S RNA
182
1
49
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  • Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses

    Letters in Applied Microbiology

    Jan 1, 2019

    Lisa F StinsonMatthew S Payne

    PMID: 30383890

    DOI: 10.1111/lam.13091

    Abstract

    Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to...read more

    Mentioned in this Paper

    Polymerase Chain Reaction
    Treatment Protocols
    RNA, Ribosomal, 16S
    Study
    Extraction
    182
    1
    49

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