Identification and sequence analysis of the genes encoding a polyketide synthase required for pyoluteorin biosynthesis in Pseudomonas fluorescens Pf-5

Gene
Brian Nowak-ThompsonJ E Loper

Abstract

Pyoluteorin is a chlorinated antifungal metabolite of mixed polyketide/amino-acid origin produced by certain strains of Pseudomonas spp., including the soil bacterium Pseudomonas fluorescens Pf-5. Sequence analysis of a gene cluster required for pyoluteorin biosynthesis by Pf-5 (Kraus, J., Loper, J., 1995. Appl. Environ. Microbiol. 61, 849-854) has identified two genes whose deduced peptide sequences exhibit characteristics of both fungal and bacterial Type I polyketide synthases (PKSs). The pyoluteorin PKS does not contain a loading domain that is typically present in bacterial Type I PKSs. Furthermore, this PKS possesses an acyltransferase domain that does not contain the conserved residues surrounding the active-site motif typically found in domains of similar function. Based on the organization of the functional domains within the pyoluteorin PKS, we propose a biosynthetic pathway analogous to non-aromatic polyketide biosynthesis within the Actinomycete bacteria that is responsible for the formation of the resorcinol moiety of pyoluteorin.

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Citations

May 24, 2005·Journal of Molecular Evolution·Aurélien GinolhacRenaud Nalin
Mar 1, 2012·The Journal of Microbiology·Hyosun Cho, Hyojeung Kang
Nov 5, 2005·Applied and Environmental Microbiology·Marion BrodhagenJoyce E Loper
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Apr 7, 2005·Chembiochem : a European Journal of Chemical Biology·Jing He, Christian Hertweck
Dec 30, 2014·Genome Announcements·Lucy M McCullyMark W Silby
Jul 11, 2006·Advances in Applied Microbiology·Karl-Heinz van PéeTobias Wage
Mar 4, 2021·Journal of the American Chemical Society·Trevor N PurdyBradley S Moore
Mar 27, 1999·Journal of Bacteriology·B Nowak-ThompsonJ E Loper
Mar 1, 2003·The New Phytologist·Thomas F C Chin-A-WoengBen J J Lugtenberg

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