Identification and sub-cellular localization of a NAD transporter in Leishmania braziliensis (Lb NDT1)

Heliyon
David S Morales HerreraMaria H Ramírez Hernández

Abstract

Nicotinamide adenine dinucleotide (NAD) is one of the central molecules involved in energy homeostasis, cellular signaling and antioxidative defense systems. Consequently, its biosynthetic pathways and transport systems are of vital importance. The nicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT), a key enzyme in the biosynthesis of NAD, is distributed in all domains of life and exhibits various isoforms in free-living organisms in contrast with intracellular parasites, which displays a single enzyme. In Leishmania braziliensis a unique cytosolic NMNAT has been reported to date and the mechanisms through which adequate levels of NAD are maintained among the different sub-cellular compartments of this parasite are unknown. Experimental evidences have related the transport of NAD to the Nucleotide Transporters (NTTs) family, whose members are located in the cytoplasmic membrane of parasitic life organisms. Additionally, the Mitochondrial Carrier Family (MCF), a group of proteins located in the membrane of internal organelles such as the mitochondria of free life organisms, has been implicated in NAD transport. Applying bioinformatics tools, the main characteristics of the MCF were found in a transporter candidat...Continue Reading

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Citations

May 13, 2021·Acta Tropica·Geovane Dias-LopesCarlos Roberto Alves

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Methods Mentioned

BETA
glycosylation
PCR
electrophoresis
chemical treatment
fluorescence microscopy

Software Mentioned

NetNGlyc
MotifFinder
PSI
NIS Elements AR
CLC Sequence Viewer
NetOGlyc
Quantity One Basic
PHYRE2
UCSF Chimera
EZ

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