Identification and subspecific differentiation of Mycobacterium scrofulaceum by automated sequencing of a region of the gene (hsp65) encoding a 65-kilodalton heat shock protein.

Journal of Clinical Microbiology
D S SwansonJ M Musser

Abstract

Mycobacterium scrofulaceum is most commonly recovered from children with cervical lymphadenitis, although it also accounts for approximately 2% of the mycobacterial infections in AIDS patients. Species assignment of M. scrofulaceum isolated by conventional techniques can be difficult and time-consuming. To develop a strategy for rapid species assignment of these organisms, a 360-bp region of the gene (hsp65) encoding a 65-kDa heat shock protein in 37 isolates from diverse sources was sequenced. Eight hsp65 alleles were identified, and these sequences formed phylogenetic clusters and lineages largely distinct from other Mycobacterium species. There was incomplete correlation between serovar designation and hsp65 allele assignment. The hsp65 data correlated strongly with the results of sequence analysis of the gene coding for 16S rRNA. Automated DNA sequencing of a 360-bp region of the hsp65 gene provides a rapid and unambiguous method for species assignment of these acid-fast organisms for diagnostic purposes.

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Citations

Nov 30, 2000·Current Infectious Disease Reports·I S Kourbeti, M J Maslow
Jul 9, 2002·The British Journal of Dermatology·Y-C LinY-H Liao
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·C Y TurenneA Kabani
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Jul 15, 2005·International Journal of Systematic and Evolutionary Microbiology·Hong KimBum-Joon Kim
Dec 22, 2019·International Wound Journal·Hakan Kunduracılar
Dec 6, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Florence GoulhenDenis Mayrand
Dec 8, 2004·Journal of Clinical Microbiology·Yansheng ZhangRichard J Wallace
Feb 13, 1999·Journal of Clinical Microbiology·H RinguetC Pierre-Audigier

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