Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)

BMC Developmental Biology
Hisayuki AmanoShinya Yamanaka

Abstract

Embryonic stem cell-specific gene (ESG) 1, which encodes a KH-domain containing protein, is specifically expressed in early embryos, germ cells, and embryonic stem (ES) cells. Previous studies identified genomic clones containing the mouse ESG1 gene and five pseudogenes. However, their chromosomal localizations or physiological functions have not been determined. A Blast search of mouse genomic databases failed to locate the ESG1 gene. We identified several bacterial artificial clones containing the mouse ESG1 gene and an additional ESG1-like sequence with a similar gene structure from chromosome 9. The ESG1-like sequence contained a multiple critical mutations, indicating that it was a duplicated pseudogene. The 5' flanking region of the ESG1 gene, but not that of the pseudogene, exhibited strong enhancer and promoter activity in undifferentiated ES cells by luciferase reporter assay. To study the physiological functions of the ESG1 gene, we replaced this sequence in ES cells with a beta-geo cassette by homologous recombination. Despite specific expression in early embryos and germ cells, ESG1-/- mice developed normally and were fertile. We also generated ESG1-/- ES cells both by a second independent homologous recombination a...Continue Reading

References

Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·P MountfordA Smith
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·V L MeinerR V Farese
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 1, 2003·Molecular and Cellular Biology·Yoshimi TokuzawaShinya Yamanaka
May 30, 2003·Nature·Kazutoshi TakahashiShinya Yamanaka
Sep 21, 2004·Oncogene·Ian Chambers, Austin Smith
Dec 29, 2004·Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Mar 25, 2005·Physiological Reviews·Anna M Wobus, Kenneth R Boheler
May 3, 2005·The Journal of Biological Chemistry·Masayoshi MaruyamaShinya Yamanaka
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·Michele Boiani, Hans R Schöler

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Citations

Apr 29, 2011·Stem Cell Reviews and Reports·Li DingFrank Buchholz
Apr 25, 2008·BMC Developmental Biology·Shinji MasuiHitoshi Niwa
Jan 13, 2009·BMC Developmental Biology·Fiona C ManserghMartin J Evans
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David-Emlyn Parfitt, Michael M Shen
Jan 29, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Piraye YurttasScott A Coonrod
Apr 25, 2008·Tissue Engineering. Part a·Dario E SepulvedaEleftherios Terry Papoutsakis
May 2, 2008·Electrophoresis·Nicolas BuhrStéphane Viville
May 30, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Noriko TsubookaShinya Yamanaka
Jan 21, 2009·Reproduction, Fertility, and Development·Tetsuya S Tanaka
Sep 24, 2009·Mechanisms of Development·Doreen SiegelWalter Knöchel

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Methods Mentioned

BETA
PCR

Software Mentioned

Blast
GeneSprings

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