PMID: 9529113Apr 7, 1998Paper

Identification and temperature regulation of Legionella pneumophila genes involved in type IV pilus biogenesis and type II protein secretion

Infection and Immunity
M R LilesNicholas P Cianciotto

Abstract

Previously, we had isolated by transposon mutagenesis a Legionella pneumophila mutant that appeared defective for intracellular iron acquisition. While sequencing in the proximity of the mini-Tn10 insertion, we found a locus that had a predicted protein product with strong similarity to PilB from Pseudomonas aeruginosa. PilB is a component of the type II secretory pathway, which is required for the assembly of type IV pili. Consequently, the locus was cloned and sequenced. Within this 4-kb region were three genes that appeared to be organized in an operon and encoded homologs of P. aeruginosa PilB, PilC, and PilD, proteins essential for pilus production and type II protein secretion. Northern blot analysis identified a transcript large enough to include all three genes and showed a substantial increase in expression of this operon when L. pneumophila was grown at 30 degrees C as opposed to 37 degrees C. The latter observation was then correlated with an increase in piliation when bacteria were grown at the lower temperature. Southern hybridization analysis indicated that the pilB locus was conserved within L. pneumophila serogroups and other Legionella species. These data represent the first isolation of type II secretory genes...Continue Reading

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Citations

Jul 9, 2002·Microbiology·Virginia AragonNicholas P Cianciotto
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Dec 16, 2004·FEMS Microbiology Letters·Jennifer Naylor, Nicholas P Cianciotto
Mar 18, 2000·Infection and Immunity·V AragonN P Cianciotto
Feb 3, 2006·Journal of Bacteriology·Kimberly A AllardNicholas P Cianciotto
Jun 6, 2007·Infection and Immunity·Christa H Chatfield, Nicholas P Cianciotto
Nov 5, 2013·International Journal of Molecular Sciences·Mena Abdel-NourCyril Guyard
Jul 3, 2004·Journal of Bacteriology·Alireza Shamaei-TousiGad Frankel
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Dec 19, 2000·Infection and Immunity·V AragonN P Cianciotto
Jun 4, 2004·Journal of Bacteriology·Maria A SöderbergNicholas P Cianciotto
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Carmen L GiltnerLori L Burrows
Nov 23, 2019·Nature Microbiology·Debnath GhosalGrant J Jensen
Oct 28, 1999·Microbiology·A B SemmlerJ S Mattick
Dec 24, 2002·Infection and Immunity·Iouri BelyiNicholas P Cianciotto
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·P H EdelsteinS Falkow
Nov 29, 2011·Environmental Microbiology·Joana CostaAntónio Veríssimo
Jul 5, 2002·Clinical Microbiology Reviews·Barry S FieldsRichard E Besser
Jun 4, 2004·Journal of Bacteriology·Jessica A Sexton, Joseph P Vogel

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