PMID: 8943082Nov 26, 1996Paper

Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants

Proceedings of the National Academy of Sciences of the United States of America
T J Bloom, J A Beavo

Abstract

Type 7 cyclic nucleotide phosphodiesterases (PDE7s) are a newly described family of enzymes having high affinity and specificity for cAMP. However, little is known about their structure, function, or regulation. We have isolated a mouse skeletal muscle cDNA representing a new alternative splice variant (PDE7A2) of the PDE7 gene. The ORF encodes a 456-amino acid protein having a predicted molecular weight of 52.4 kDa. The 5' end of the mouse PDE7A2 is divergent from the 5' end of the human PDE7A1 sequence and is more hydrophobic. A comparison of the 5' ends of the two cDNA clones with human genomic sequence indicates that they represent alternate splice products rather than species variation. RNase protection analysis of several mouse tissues indicates that PDE7 is expressed widely with highest levels in skeletal muscle. HPLC fractionation and Western blot analysis of two human lymphocyte T-cell lines shows that an unknown PDE activity described by Ichimura and Kase [Ichimura, M. & Kase, H. (1993) Biochem. Biophys. Res. Commun. 193, 985-990] is most likely to be PDE7A1. A single immunoreactive band of approximately 55 kDa, which comigrates with PDE7A1, is seen in fractions of the HPLC profile containing this activity suggesting ...Continue Reading

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M StrathmannM J Palazzolo
Jun 1, 1989·Journal of Autonomic Pharmacology·C D Nicholson, R Wilke
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 30, 1993·Biochemical and Biophysical Research Communications·M Ichimura, H Kase

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Citations

May 18, 2000·Biochemical and Biophysical Research Communications·T SasakiK Omori
Mar 27, 1998·Cell Biochemistry and Biophysics·R HoffmannP Engels
Oct 24, 2007·Applied Microbiology and Biotechnology·Renu MalikKulvinder Singh Saini
Aug 5, 1999·Experimental Gerontology·H GlossmannG Bartsch
May 1, 2001·Bioorganic & Medicinal Chemistry Letters·M J BarnesR J Watson
Sep 16, 1998·Current Opinion in Chemical Biology·M J Perry, G A Higgs
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lingzhi ZhangPaul A Insel
Jun 28, 2005·Nucleic Acids Research·Shobhit GuptaStefan A Haas
Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·M FuhrmannN Suttorp
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shinji WakabayashiKunio Takaoka
Oct 10, 2009·PloS One·Jolena N Fleming-WaddellChristopher A Bidwell
Oct 12, 2000·Biochemical and Biophysical Research Communications·P WangM M Billah
Dec 19, 2007·Asian Journal of Andrology·F DimitriadisN Sofikitis
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S H SoderlingJ A Beavo
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A GlavasJ A Beavo
Jun 30, 2001·Journal of Neuroimmunology·K MakhloufS J Khoury
Dec 25, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Takayuki MatsumotoKatsuo Kamata
Apr 28, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Marcia A WheelerRobert M Weiss
May 11, 2013·Cellular and Molecular Life Sciences : CMLS·E M Medina-RodríguezF de Castro
Apr 11, 2013·Expert Opinion on Drug Discovery·Maliheh SafaviMohammad Abdollahi
Feb 7, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Daniel I PérezCarlo Bertucci
Nov 4, 2011·Current Opinion in Pharmacology·Isaac LevyConstantine A Stratakis
Feb 6, 2010·European Journal of Pharmacology·Megumi GotoYoshiaki Fukuda
Nov 11, 2008·International Immunopharmacology·Kumiko Kadoshima-YamaokaTakashi Nishimura
Jan 26, 2007·European Journal of Pharmacology·Satoshi YamamotoYasuaki Shimizu
Apr 28, 2005·Molecular Reproduction and Development·Rhona W Baxendale, Lynn R Fraser
Jan 30, 2007·Animal Genetics·J N Fleming-WaddellC A Bidwell
Feb 7, 2006·European Journal of Pharmacology·Kian Fan Chung
Jan 24, 2006·Pulmonary Pharmacology & Therapeutics·N A JonesC P Page

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