Identification and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry characterization of biosurfactants, including a new surfactin, isolated from oil-contaminated environments

Microbial Biotechnology
Glaci V MoroMarilene H Vainstein

Abstract

Biosurfactant-producing bacteria were isolated from samples collected in areas contaminated with crude oil. The isolates were screened for biosurfactant production using qualitative drop-collapse test, oil-spreading and emulsification assays, and measurement of their tensoactive properties. Five isolates tested positive for in the screening experiments and displayed decrease in the surface tension below 30 mN m-1 . The biosurfactants produced by these isolates were further investigated and their molecular identification revealed that they are bacteria related to the Bacillus genus. Additionally, the biosurfactants produced were chemically characterized via UHPLC-HRMS experiments, indicating the production of surfactin homologues, including a new class of these molecules.

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Citations

Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anita KecskemétiAndrás Szekeres
Oct 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Attila BartalAndrás Szekeres
Mar 7, 2021·International Journal of Molecular Sciences·Ana B MoldesJosé M Cruz
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz JanekZbigniew Lazar
Aug 13, 2021·Scientific Reports·Albert D PatiñoJavier Gómez-León
Sep 18, 2021·Analytical Chemistry·Daniel PetrasMingxun Wang
Sep 28, 2021·Mass Spectrometry Reviews·Raffaella PascaleGiuliana Bianco

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
Cluster
MS
Bruker
Data Analysis
Cytoscape
GNPS

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