Identification and Unusual Properties of the Master Regulator FNR in the Extreme Acidophile Acidithiobacillus ferrooxidans

Frontiers in Microbiology
Héctor OsorioD S Holmes

Abstract

The ability to conserve energy in the presence or absence of oxygen provides a metabolic versatility that confers an advantage in natural ecosystems. The switch between alternative electron transport systems is controlled by the fumarate nitrate reduction transcription factor (FNR) that senses oxygen via an oxygen-sensitive [4Fe-4S]2+ iron-sulfur cluster. Under O2 limiting conditions, FNR plays a key role in allowing bacteria to transition from aerobic to anaerobic lifestyles. This is thought to occur via transcriptional activation of genes involved in anaerobic respiratory pathways and by repression of genes involved in aerobic energy production. The Proteobacterium Acidithiobacillus ferrooxidans is a model species for extremely acidophilic microorganisms that are capable of aerobic and anaerobic growth on elemental sulfur coupled to oxygen and ferric iron reduction, respectively. In this study, an FNR-like protein (FNRAF) was discovered in At. ferrooxidans that exhibits a primary amino acid sequence and major motifs and domains characteristic of the FNR family of proteins, including an effector binding domain with at least three of the four cysteines known to coordinate an [4Fe-4S]2+ center, a dimerization domain, and a DNA b...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·P J Kiley, W S Reznikoff
Dec 1, 1987·Journal of General Microbiology·S Spiro, J R Guest
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N KhoroshilovaP J Kiley
Jul 15, 1993·Biochemical and Biophysical Research Communications·J FujinagaJ Meyer
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·N KhoroshilovaP J Kiley
Dec 31, 1997·FEBS Letters·P A JordanJ Green
Jun 23, 1998·The Journal of Biological Chemistry·G B McGaugheyA K Rappé
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·C V PopescuP J Kiley
Oct 3, 2001·The Journal of Biological Chemistry·L J Moore, P J Kiley
Nov 18, 2004·Journal of Bacteriology·Victoria R SuttonPatricia J Kiley
Jul 1, 1991·Applied and Environmental Microbiology·J T PronkJ G Kuenen
Sep 9, 2006·The Journal of Biological Chemistry·Laura J MoorePatricia J Kiley
Feb 13, 2007·Journal of Bacteriology·Erin L Mettert, Patricia J Kiley
Jun 11, 2008·Methods in Molecular Biology·Narayanan EswarAndrej Sali
Feb 28, 2009·Current Opinion in Microbiology·Jeffrey GreenNick E LeBrun
Mar 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Adrian J JervisJeffrey Green
Nov 7, 2009·Methods in Enzymology·Aixin Yan, Patricia J Kiley
Nov 18, 2009·The Journal of Biological Chemistry·James EdwardsJames W B Moir
Sep 14, 2010·Extremophiles : Life Under Extreme Conditions·Craig Baker-AustinMark Dopson
Feb 5, 2011·Current Opinion in Chemical Biology·Angela S Fleischhacker, Patricia J Kiley
Nov 5, 2011·Environmental Microbiology·Violaine Bonnefoy, David S Holmes
Nov 22, 2011·Antioxidants & Redox Signaling·Emilio BuenoMaria J Delgado
Apr 19, 2012·Environmental Microbiology·Mark Dopson, D Barrie Johnson
Dec 1, 2012·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jan 29, 2013·Applied and Environmental Microbiology·Héctor OsorioDavid S Holmes
May 31, 2013·Applied Microbiology and Biotechnology·Mario VeraWolfgang Sand
Jul 3, 2013·Journal of Molecular Biology·Ivan L BudyakLila M Gierasch
Jul 24, 2013·Applied Microbiology and Biotechnology·Corale L Brierley, James A Brierley
Sep 26, 2013·Current Opinion in Structural Biology·Jane S RichardsonDavid C Richardson
Oct 15, 2013·FEMS Microbiology Letters·Sabrina Hedrich, D Barrie Johnson
Apr 8, 2014·Nature Chemical Biology·Stefan FrielingsdorfPatrick Scheerer
Aug 12, 2014·Applied Microbiology and Biotechnology·Mark Dopson, David S Holmes
May 15, 2015·The Journal of Biological Chemistry·Susan A IbrahimJeffrey Green

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

Graphpad Prism
Modeler
BlastP
Swiss
! Validation Server
ADIT
Model
Clustal Omega

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.