Identification and validation a TGF-β-associated long non-coding RNA of head and neck squamous cell carcinoma by bioinformatics method

Journal of Translational Medicine
Teng HuangXia He

Abstract

The role of transforming growth factorβ (TGF-β)-induced tumor progression in advanced malignancy is well established, but the involvement of long non-coding RNAs (lncRNAs) in TGF-β signaling remains unclear. This study aimed to identify TGF-β-associated lncRNAs in head and neck squamous cell carcinoma (HNSCC). Expression profiling of lncRNAs was obtained using Gene Expression Omnibus and The Cancer Genome Atlas. Real-time quantitative PCR was used to analyze the expression of EPB41L4A-AS2 in HNSCC cell line. We used bioinformatics resources (DAvID) to conduct Gene Ontology biological processes and KEGG pathways at the significant level. Wound healing assay, cell migration and invasion assays, were used to examine the effects of EPB41L4A-AS2 on tumor cell metastasis in vivo. Protein levels of EPB41L4A-AS2 targets were determined by western blot. A novel TGF-β-associated lncRNA, EPB41L4A-AS2, was found downregulated by TGF-β and associated with invasion and metastasis. The relationship of EPB41L4A-AS2 with the clinicopathological features and prognosis of HNSCC patients was evaluated. Bioinformatic analyses revealed that EPB41L4A-AS2 may be involved in processes associated with the tumor-associated signaling pathway, especially t...Continue Reading

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Citations

Jun 3, 2020·Cancer Science·Panagiotis Papoutsoglou, Aristidis Moustakas
Apr 12, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yu-Gang WangBo Zhai
May 10, 2019·Journal of Cellular Biochemistry·Seyed Ali MirhosseiniJafar Amani
Nov 13, 2020·Cancer Biotherapy & Radiopharmaceuticals·Guo-Sheng LiHong-Yu Wei
Mar 9, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Wenxiao JiangXueqiong Zhu

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Datasets Mentioned

BETA
GSE59652
GSE54800

Methods Mentioned

BETA
RNASeq
transfection

Software Mentioned

GraphPad Prism
TCGA RNASeqV2
miRanda
Cytoscape
SPSS

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