Identification and validation of potential key long noncoding RNAs in sorafenib-resistant hepatocellular carcinoma cells

PeerJ
Manya WuXiaoling Luo

Abstract

As the first-line treatment, sorafenib has been used for advanced hepatocellular carcinoma (HCC), but the chemoresistance commonly restricts to the clinical efficiency. In this study, we intend to investigate the genome-wide expression pattern of long noncoding RNAs (lncRNAs) in sorafenib-resistant HCC. Herein, we identified thousands of differentially expressed lncRNAs in sorafenib-resistant HCC cells by high-throughput sequencing compared to the parental. Besides, based on GO (Gene Ontology) term enrichment analysis, these differentially expressed lncRNAs are mainly related to binding and catalytic activity and biological regulation of metabolic processes in both the sorafenib-resistant Huh7 cells (Huh7-S) and sorafenib-resistant HepG2 cells (HepG2-S) compared to the parental cells. Moreover, when analyzed by KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway, the differentially expressed genes were significantly related to the tight junction. Among them, the expression of TCONS_00284048 and TCONS_00006019 was consistently up-regulated in sorafenib-resistant HCC cell lines, whereas when either was knocked down, the sensitivity of Huh7-S and HepG2-S cells to sorafenib was increased. Taken together, our data demonstrate tha...Continue Reading

References

Jan 27, 2000·Cell·D Hanahan, R A Weinberg
May 16, 2003·Pflügers Archiv : European journal of physiology·Ernest M Wright, Eric Turk
Aug 13, 2005·Anti-cancer Drugs·Daniel Morgensztern, Howard L McLeod
Jul 25, 2008·The New England Journal of Medicine·Josep M LlovetUNKNOWN SHARP Investigators Study Group
Jul 20, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fu YangShu-han Sun
Aug 9, 2012·European Journal of Medicinal Chemistry·Kuen-Feng ChenChung-Wai Shiau
Mar 20, 2013·Molecular Aspects of Medicine·Ernest M Wright
Apr 24, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jessica LoTerence Kin Wah Lee
Oct 19, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ben YueDongwang Yan
Mar 9, 2017·Journal of Cancer Research and Clinical Oncology·Ji-Nan XiaoJin-Hua Jiang
Mar 12, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinxia LiuRunzhou Ni
Jan 24, 2018·Inflammatory Bowel Diseases·Yael HabermanLee A Denson
Sep 15, 2018·Journal of Cellular Biochemistry·Xiaoliang ChenChunshu Jia

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
RNA Assay
Assay
RNA-seq
PCR
Transfections
transfection

Software Mentioned

Cutadapt
FastQC
EdgeR
DESeq2
Bcl2fastq
DESeq
GO
Stringtie
Bioconductor
TermFinder

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.