Identification by a differential proteomic approach of the induced stress and redox proteins by resveratrol in the normal and diabetic rat heart

Journal of Cellular and Molecular Medicine
D H W DekkersDipak K Das

Abstract

A recent study showed cardioprotective effects of resveratrol on the diabetic heart. The present study sought to compare the protein profiles of the normal versus diabetic hearts after resveratrol treatment using differential proteomic analysis. Rats were randomly divided into two groups: control and diabetic. Both groups of rats were fed resveratrol (2.5 mg/kg/day) for 7 days, and then the rats were sacrificed, hearts were isolated and cytoplasmic fraction from left ventricular tissue was collected to carry out proteomic profiling as well as immunoblotting. Compared to normal hearts, diabetic hearts show increased myocardial infarct size and cardiomy-ocyte apoptosis upon ex vivo global ischaemia of 30 min. followed by 2 hrs of reperfusion. Resveratrol reduced infarct size and apop-totic cell death for both the groups, but the extent of infarct size and apoptosis remained higher for the diabetic group compared to the normal group. The left ventricular cytoplasmic proteins were analysed by 2D-DIGE and differentially displayed bands were further analysed by nano Liquid Chromatography-Mass Spectroscopy (LC-MS/MS). The results showed differential regulation of normal versus diabetic hearts treated with resveratrol of many proteins ...Continue Reading

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Citations

Mar 4, 2010·Journal of Proteome Research·Lianna LiBarbara Sherry
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leonie F WaandersMatthias Mann
Dec 9, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Radha AnanthakrishnanRavichandran Ramasamy
Jul 9, 2014·Pharmacology & Therapeutics·Giacinta GuariniMario Marzilli
Mar 20, 2010·Heart Failure Reviews·Dipak K DasDiptarka Ray
Mar 15, 2011·Heart Failure Reviews·Dipak K DasDiptarka Ray
Dec 31, 2010·Dose-response : a Publication of International Hormesis Society·Subhendu MukherjeeDipak K Das
Mar 11, 2018·Clinical Oral Investigations·Sven-Olav PabelAnnette Wiegand
Dec 17, 2019·Acta Physiologica·Marina Makrecka-KukaTerje S Larsen
Mar 11, 2020·Antioxidants & Redox Signaling·Christian Cadeddu DessalviGiuseppe Mercuro
Jul 28, 2016·Expert Review of Proteomics·José TuñónJesús Egido
Jul 19, 2021·Basic Research in Cardiology·Ioanna AndreadouRainer Schulz

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Methods Mentioned

BETA
proteomic profiling
protein assay
proteomic

Software Mentioned

SPSS
DeCyder
Mascot Distiller
Matrix Science
Mascot
Scion Image
NIH

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis