Abstract
The polymerase chain reaction-based differential display method (DDRT-PCR) was used to identify mRNAs differentially expressed during the maturation of human CD34+ progenitor cells stimulated to differentiate in vitro towards granulomonocytic or erythroid lineages with a mixture of hemopoietins (kit ligand + interleukin 3 + GM-CSF in the absence or presence of erythropoietin, respectively). Three cDNA transcripts (B32, B41, and B56) display differential expression during cytokine-induced maturation of CD34+ cells. These clones have no homology with already-described sequences. Primer extension cofirmed the presence of the corresponding mRNA. The levels of mRNA corresponding to B32 are enhanced in the later phases of the granulomonocytic as well as in the erythroid differentiation of CD34+ cells. The mRNA identified by B41 was induced by a late stage in only granulomonocytic differentiation of CD34+ cells. The mRNA corresponding to B56 was instead present in nonstimulated CD34+ cells, declined in the early stages of differentiation, and reappeared at later stages in cells treated with both combinations of cytokines. Expression of these genes was detected in a number of acute myelogenous leukemias, as well as in some leukemic cel...Continue Reading
References
Jan 1, 1991·Leukemia Research·C J PuntG E Staal
Jan 25, 1991·Cell·C L SawyersO N Witte
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Sep 25, 1994·Nucleic Acids Research·B P Sokolov, D J Prockop
Jan 6, 1995·Science·A C BerardiD T Scadden
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·D OrlicD M Bodine
Apr 1, 1995·Current Opinion in Immunology·P Liang, A B Pardee
Oct 27, 1995·The Journal of Biological Chemistry·L DongS Jaken
Aug 1, 1995·Trends in Biochemical Sciences·M S Boguski
Mar 10, 1995·The Journal of Biological Chemistry·S MannoN Mohandas
Jun 1, 1994·The Biochemical Journal·C SjölinC Dahlgren
Jul 11, 1993·Nucleic Acids Research·P LiangA B Pardee
Sep 11, 1993·Nucleic Acids Research·D BauerM Strauss
Apr 1, 1996·Annals of Hematology·M J KroefJ E Landegent
Jun 15, 1995·The Biochemical Journal·G MorroneS Venuta
Citations
Feb 28, 2002·Gene·Jiachang ZhangGriffin P Rodgers
Nov 25, 2000·Journal of Hematotherapy & Stem Cell Research·Z B XiaL Lu
Jun 16, 2005·Stem Cells·Martina KomorWolf-K Hofmann
Jan 29, 2005·Experimental Hematology·Alan G RosmarinKaren K Resendes
May 29, 2002·Oncogene·Diane S Krause
Jun 9, 2005·Oncogene·Jens Claus HahneNicolas Wernert
Dec 8, 2004·Oncogene·Kathryn E HentgesMonica J Justice
Sep 29, 2006·Folia Microbiologica·I SchréterA Gocalová
May 19, 2009·Virus Genes·A DencsM Takács
Jun 21, 2006·Molecular and Cellular Biochemistry·Xiao-Fang HuoJun-Wu Zhang