Identification by DNA microarray of genes involved in Candida albicans-treated gingival epithelial cells

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Tonami IkutaYoshimitsu Abiko

Abstract

Oral epithelial cells significantly influence host inflammatory responses against Candida albicans in oropharyngeal candidiasis. Pro-inflammatory cytokines function as an early innate immune system mediator during C. albicans infection in oral epithelial cells. We sought to elucidate the pattern of the molecular mechanisms governing the human gingival epithelial cells (HGECs) to C. albicans infection likely involve multiple converging signal transduction pathways. Primary HGECs were cultured with C. albicans ATCC90029. Total RNA was extracted after 8 h of infection and monitored mRNA levels using Affymetrix GeneChip (Human Genome U133 plus 2.0 Array, 48 000 genes). GeneChip data was analyzed by GeneSpring software and Ingenuity Pathway Analysis system. Reverse transcription polymerase chain reaction (RT-PCR), real-time RT-PCR and immunohistochemistry were used to investigate gene expression changes. The differentially expressed genes represented functions as diverse as immune response and inflammatory disease. IL-8, ICAM-1 and Cox-2 showed a greater than two fold change in expression relative to those in control cells. Altered mRNA levels in GeneChip analysis were confirmed by RT-PCR and real-time RT-PCR. Stronger immunoreactiv...Continue Reading

References

Dec 11, 1992·Science·A E KochR M Strieter
Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D J Smith, M A Taubman
Feb 1, 1987·Journal of Oral Pathology·C M Allen, F M Beck
Feb 1, 1987·Journal of Periodontology·S GonzálezF R Saglie
Oct 1, 1995·Journal of Medical Microbiology·B el MoudniJ L Jacquemin
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·N E WickershamJ W Christman
Feb 1, 1993·Immunology Today·J D Bos, M L Kapsenberg
Nov 5, 1997·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·L R EversoleN Pimpinelli
Apr 3, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C J Fichtenbaum, W G Powderly
Jul 9, 1999·The Journal of Infectious Diseases·M GottfredssonJ R Perfect
Sep 4, 1999·FEMS Microbiology Letters·M H RodierJ L Jacquemin
Feb 12, 2000·Fungal Genetics and Biology : FG & B·M K Hostetter
Aug 9, 2001·Journal of Clinical Periodontology·A H ReynaudP Gjermo
Mar 29, 2002·The Journal of Investigative Dermatology·Martin SchallerHans C Korting
Aug 6, 2002·Current Opinion in Microbiology·Neil A R GowFrank C Odds
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aihua LiRakesh K Singh
Apr 2, 2003·Microbial Pathogenesis·A Dongari-Bagtzoglou, H Kashleva
Dec 31, 2003·Advances in Applied Microbiology·Chantal Fradin, Bernhard Hube
Dec 25, 2004·International Journal of Oral and Maxillofacial Surgery·C M Scott, S R Flint
Apr 22, 2005·Mycoses·Maria BelaziDimitrios Karamitsos
Aug 6, 2005·Journal of Clinical Microbiology·Luis Octavio Sánchez-VargasGuillermo Quindós
Oct 26, 2005·Journal of Dental Research·A Dongari-Bagtzoglou, P L Fidel
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Jodi Marie SaunusCamile Selim Farah

❮ Previous
Next ❯

Citations

Feb 26, 2016·Frontiers in Microbiology·S P SmeekensM G Netea
Sep 22, 2015·Nature Reviews. Immunology·Mihai G NeteaFrank L van de Veerdonk

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.