Identification by monoclonal antibodies of T-cell subpopulations activated by 12-O-tetradecanoylphorbol-13-acetate (TPA)

Immunology Letters
J H KaplanS H Lockwood

Abstract

Two major T-cell subpopulations obtained from human peripheral blood were studied their capacity to be activated by the tumor promoting agent, 12-O-tetradecanoylphorbol-13-acetate (TPA). The subpopulations were identified and separated from each other by their reactivity either to the monoclonal antibody, OKT4 or OKT8, followed by complement-dependent lysis. The fraction enriched in T8+ cells showed approximately a 1.2-2.0-fold greater proliferative response to TPA (20 ng/ml) than did the T4+ cell subset. Optimal activation required the presence of monocytes. Comparison of the mitotic activity showed that the sum of each T-cell subset was less than a mixture of the two. This indicates either a unilateral or cooperative interaction of mitogenesis in the presence of TPA.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Feb 27, 1975·The New England Journal of Medicine·H H FudenbergD Robbins
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T W ChangG Goldstein
Apr 1, 1980·The Journal of Experimental Medicine·E L ReinherzS F Schlossman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman

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