Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes

Proceedings of the National Academy of Sciences of the United States of America
Maddalena FratelliPietro Ghezzi

Abstract

Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the redox status. The aim of this study was to identify the proteins of T cell blasts that undergo glutathionylation under oxidative stress. To this purpose, we radiolabeled cellular glutathione with (35)S, exposed T cells to oxidants (diamide or hydrogen peroxide), and performed nonreducing, two-dimensional electrophoresis followed by detection of labeled proteins by phosphorimaging and their identification by mass spectrometry techniques. We detected several proteins previously not recognized to be glutathionylated, including cytoskeletal proteins (vimentin, myosin, tropomyosin, cofilin, profilin, and the already known actin), enzymes (enolase, aldolase, 6-phosphogluconolactonase, adenylate kinase, ubiquitin-conjugating enzyme, phosphoglycerate kinase, triosephosphate isomerase, and pyrophosphatase), redox enzymes (peroxiredoxin 1, protein disulfide isomerase, and cytochrome c oxidase), cyclophilin, stress proteins (HSP70 and HSP60), nucleophosmin, transgelin, galectin, and fatty acid binding protein. Based on the presence of several prot...Continue Reading

References

Jan 1, 1984·Methods in Enzymology·H F Gilbert
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·F J StaalL A Herzenberg
Sep 1, 1995·The American Journal of Physiology·C M Padgett, A R Whorton
Feb 20, 1995·Archives of Biochemistry and Biophysics·Y ZhangR W Gracy
Feb 20, 1996·Biochemistry·D A DavisR L Levine
Nov 14, 1997·The Journal of Biological Chemistry·J Jahngen-HodgeA Taylor
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D PetersonC Waltenbaugh
May 6, 1998·European Journal of Clinical Investigation·A J van der VenJ W van der Meer
Feb 23, 1999·Methods in Molecular Biology·E Gianazza
Aug 27, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P KlattS Lamas
Oct 26, 1999·Biochemical and Biophysical Research Communications·A NakamuraS Goto
Apr 29, 2000·Electrophoresis·K Gevaert, J Vandekerckhove
Jun 15, 2000·The Journal of Biological Chemistry·E CabiscolJ Ros
Aug 29, 2000·Journal of Muscle Research and Cell Motility·I Dalle-DonneR Rossi
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder

❮ Previous
Next ❯

Citations

Aug 5, 2003·BioFactors·Eszter PappPéter Csermely
Dec 30, 2004·Electrophoresis·Gary Guishan XiaoJoseph A Loo
Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
Sep 29, 2004·Histochemistry and Cell Biology·Leni Moldovan, Nicanor I Moldovan
Oct 6, 2006·Amino Acids·E GianazzaI Miller
Apr 25, 2012·Journal of Inherited Metabolic Disease·Niels GregersenJohan Palmfeldt
Apr 14, 2010·Cancer Metastasis Reviews·Giovambattista PaniPaola Chiarugi
Nov 8, 2006·Photosynthesis Research·Laure MicheletStéphane D Lemaire
Nov 8, 2006·Photosynthesis Research·Valérie Noguera-MazonJean-Marc Lancelin
Mar 3, 2004·Archives of Biochemistry and Biophysics·Regina Brigelius-FlohéGaby-Fleur Böl
Nov 22, 2005·Archives of Biochemistry and Biophysics·Suryakant K NitureKalkunte S Srivenugopal
May 25, 2005·Biochemical and Biophysical Research Communications·Ylva Hamnell-PammentIan A Cotgreave
Apr 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Valerio NobiliFiorella Piemonte
May 14, 2005·Free Radical Biology & Medicine·Isabella Dalle-DonneRanieri Rossi
Mar 31, 2004·Toxicology and Applied Pharmacology·Pramod K AllaniManisha Sonee
Oct 15, 2013·Trends in Biochemical Sciences·Ryan J MaillouxMary-Ellen Harper
Jun 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anna PastoreFiorella Piemonte
May 22, 2003·Progress in Neurobiology·Gert LubecMichael Fountoulakis
May 14, 2003·Experimental Gerontology·Christian Schöneich
Nov 11, 2003·Free Radical Biology & Medicine·Jaroslaw KanskiChristian Schöneich
Nov 11, 2003·Free Radical Biology & Medicine·Isabella Dalle-DonneAldo Milzani
Sep 5, 2003·Journal of the American Society for Mass Spectrometry·Sabrina Laugesen, Peter Roepstorff
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Aug 12, 2010·Biochemistry·Stefano M MarinoVadim N Gladyshev
Jul 8, 2009·Biochemistry·Gresin O Pizarro, Ozgur Ogut
Aug 19, 2008·Journal of Proteome Research·Cexiong FuHong Li
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lars I LeichertUrsula Jakob
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
Jun 30, 2009·The Journal of Biological Chemistry·Ji Won ParkP Boon Chock
Oct 13, 2010·The Journal of Biological Chemistry·Jerome GarciaEnrique Cadenas
Oct 7, 2011·The Journal of Biological Chemistry·Nicolas BrandesUrsula Jakob
Jan 28, 2009·The Journal of Biological Chemistry·Ikuo SatoNaoki Takaya
Jul 19, 2013·The Journal of Biological Chemistry·Young-Mi Go, Dean P Jones
Dec 14, 2011·Toxicological and Environmental Chemistry·Matthew Garrecht, David W Austin
Jul 26, 2003·Antioxidants & Redox Signaling·Nikola J CostaMichael P Murphy
Apr 30, 2003·Antioxidants & Redox Signaling·Regina Brigelius-FlohéKerstin Schnurr
Jul 7, 2005·Antioxidants & Redox Signaling·Pietro GhezziMaddalena Fratelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.