Abstract
Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the redox status. The aim of this study was to identify the proteins of T cell blasts that undergo glutathionylation under oxidative stress. To this purpose, we radiolabeled cellular glutathione with (35)S, exposed T cells to oxidants (diamide or hydrogen peroxide), and performed nonreducing, two-dimensional electrophoresis followed by detection of labeled proteins by phosphorimaging and their identification by mass spectrometry techniques. We detected several proteins previously not recognized to be glutathionylated, including cytoskeletal proteins (vimentin, myosin, tropomyosin, cofilin, profilin, and the already known actin), enzymes (enolase, aldolase, 6-phosphogluconolactonase, adenylate kinase, ubiquitin-conjugating enzyme, phosphoglycerate kinase, triosephosphate isomerase, and pyrophosphatase), redox enzymes (peroxiredoxin 1, protein disulfide isomerase, and cytochrome c oxidase), cyclophilin, stress proteins (HSP70 and HSP60), nucleophosmin, transgelin, galectin, and fatty acid binding protein. Based on the presence of several prot...Continue Reading
References
Sep 11, 1992·Science·C HwangH F Lodish
Feb 2, 1989·Nature·G FischerF X Schmid
Oct 1, 1989·Neuropharmacology·M UkaiT Kameyama
Feb 7, 1989·Biochemistry·W Y ChanP K Chan
Aug 15, 1970·Nature·U K Laemmli
May 1, 1982·Journal of General Microbiology·R Lahti, M Suonpää
Jan 1, 1984·Methods in Enzymology·H F Gilbert
Sep 1, 1983·Biochemical Pharmacology·R BrigeliusH Sies
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·F J StaalL A Herzenberg
Sep 1, 1995·The American Journal of Physiology·C M Padgett, A R Whorton
Feb 20, 1995·Archives of Biochemistry and Biophysics·Y ZhangR W Gracy
Feb 20, 1996·Biochemistry·D A DavisR L Levine
Sep 12, 1997·The Journal of Biological Chemistry·F ShangA Taylor
Nov 14, 1997·The Journal of Biological Chemistry·J Jahngen-HodgeA Taylor
Jan 24, 1998·Biochemical and Biophysical Research Communications·I A Cotgreave, R G Gerdes
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D PetersonC Waltenbaugh
Mar 21, 1998·Electrophoresis·K GevaertJ Vandekerckhove
May 6, 1998·European Journal of Clinical Investigation·A J van der VenJ W van der Meer
Feb 23, 1999·Methods in Molecular Biology·E Gianazza
Apr 2, 1999·Cell·F Aslund, J Beckwith
Aug 27, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P KlattS Lamas
Oct 26, 1999·Biochemical and Biophysical Research Communications·A NakamuraS Goto
Dec 28, 1999·Electrophoresis·D N PerkinsJ S Cottrell
Apr 29, 2000·Electrophoresis·K Gevaert, J Vandekerckhove
Jun 15, 2000·The Journal of Biological Chemistry·E CabiscolJ Ros
Aug 29, 2000·Journal of Muscle Research and Cell Motility·I Dalle-DonneR Rossi
Sep 22, 2000·Biochemistry·D M SullivanT Finkel
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder
Jun 8, 2001·The Journal of Biological Chemistry·T OkamotoH Maeda
Jul 18, 2001·The Journal of Biological Chemistry·E MicletF Duffieux
Feb 14, 2002·Molecular Immunology·Pietro GhezziLeonard A Herzenberg
Citations
Aug 5, 2003·BioFactors·Eszter PappPéter Csermely
Dec 30, 2004·Electrophoresis·Gary Guishan XiaoJoseph A Loo
Mar 20, 2003·Mass Spectrometry Reviews·Filippo RusconiDanièle Praseuth
Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
Sep 29, 2004·Histochemistry and Cell Biology·Leni Moldovan, Nicanor I Moldovan
Oct 6, 2006·Amino Acids·E GianazzaI Miller
May 25, 2010·Molecules and Cells·Il-Sup KimHo-Sung Yoon
Dec 23, 2008·Marine Biotechnology·E Salas-LeitonJ Alhama
Apr 25, 2012·Journal of Inherited Metabolic Disease·Niels GregersenJohan Palmfeldt
Apr 14, 2010·Cancer Metastasis Reviews·Giovambattista PaniPaola Chiarugi
Nov 8, 2006·Photosynthesis Research·Laure MicheletStéphane D Lemaire
Nov 8, 2006·Photosynthesis Research·Valérie Noguera-MazonJean-Marc Lancelin
Mar 3, 2004·Archives of Biochemistry and Biophysics·Regina Brigelius-FlohéGaby-Fleur Böl
Nov 22, 2005·Archives of Biochemistry and Biophysics·Suryakant K NitureKalkunte S Srivenugopal
May 5, 2005·Aquatic Toxicology·Brian McDonaghDavid Sheehan
May 25, 2005·Biochemical and Biophysical Research Communications·Ylva Hamnell-PammentIan A Cotgreave
Apr 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Valerio NobiliFiorella Piemonte
May 14, 2005·Free Radical Biology & Medicine·Isabella Dalle-DonneRanieri Rossi
Jun 4, 2005·Brain Research. Molecular Brain Research·H Fai PoonD Allan Butterfield
Mar 31, 2004·Toxicology and Applied Pharmacology·Pramod K AllaniManisha Sonee
Oct 15, 2013·Trends in Biochemical Sciences·Ryan J MaillouxMary-Ellen Harper
Jun 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anna PastoreFiorella Piemonte
May 22, 2003·Progress in Neurobiology·Gert LubecMichael Fountoulakis
Dec 10, 2002·Experimental Gerontology·Sylvie PoggioliBertrand Friguet
May 14, 2003·Experimental Gerontology·Christian Schöneich
Dec 25, 2002·Free Radical Biology & Medicine·I Dalle-DonneA Milzani
Nov 11, 2003·Free Radical Biology & Medicine·Jaroslaw KanskiChristian Schöneich
Nov 11, 2003·Free Radical Biology & Medicine·Isabella Dalle-DonneAldo Milzani
Sep 5, 2003·Journal of the American Society for Mass Spectrometry·Sabrina Laugesen, Peter Roepstorff
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Aug 12, 2010·Biochemistry·Stefano M MarinoVadim N Gladyshev
Dec 1, 2011·Biochemistry·Hameed M S Shahul, Siddhartha P Sarma
Jul 8, 2009·Biochemistry·Gresin O Pizarro, Ozgur Ogut
Nov 28, 2012·Chemical Reviews·Angela BachiAndrea Scaloni
Apr 12, 2011·Journal of Proteome Research·Christina L Takanishi, Matthew J Wood
Aug 19, 2008·Journal of Proteome Research·Cexiong FuHong Li
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lars I LeichertUrsula Jakob
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
Jun 30, 2009·The Journal of Biological Chemistry·Ji Won ParkP Boon Chock
Oct 13, 2010·The Journal of Biological Chemistry·Jerome GarciaEnrique Cadenas
Oct 7, 2011·The Journal of Biological Chemistry·Nicolas BrandesUrsula Jakob
Jul 10, 2008·The Journal of Biological Chemistry·Thomas R HurdMichael P Murphy
Jan 28, 2009·The Journal of Biological Chemistry·Ikuo SatoNaoki Takaya
Jul 19, 2013·The Journal of Biological Chemistry·Young-Mi Go, Dean P Jones
Dec 14, 2011·Toxicological and Environmental Chemistry·Matthew Garrecht, David W Austin
Jul 26, 2003·Antioxidants & Redox Signaling·Nikola J CostaMichael P Murphy
Apr 30, 2003·Antioxidants & Redox Signaling·Regina Brigelius-FlohéKerstin Schnurr
Feb 12, 2005·Antioxidants & Redox Signaling·Melissa D SheltonJohn J Mieyal
Jul 7, 2005·Antioxidants & Redox Signaling·Feng Chu, Catherine A O'Brian
Jul 7, 2005·Antioxidants & Redox Signaling·Pietro GhezziMaddalena Fratelli