Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis

Journal of Insect Physiology
Jatuporn TungjitwitayakulSho Sakurai

Abstract

Two insect storage proteins, OfSP1 (75 kDa) and OfSP2 (72 kDa), were purified using three different chromatographies from the hemolymph of Omphisa fuscidentalis larvae during diapause, and their genes were cloned. OfSP1 and OfSP2 concentrations in the hemolymph were high during diapause. During pupation, OfSP1 levels decreased in the male hemolymph and disappeared from the female hemolymph. OfSP1 and OfSP2 mRNA levels in the fat bodies were low during the third instar, but increased greatly during the fourth and fifth larval instars. During diapause, mRNA expression continued at a lower level than during the feeding period. The injection of 20-hydroxyecdysone (20E) into diapausing larvae caused an increase in OfSP1 and OfSP2 mRNA levels 2-3 days post-injection, followed by a decrease in expression until pupation, which occurred 2-4 days thereafter. When larvae were treated with juvenile-hormone analog (JHA), OfSP1 and OfSP2 mRNA levels gradually decreased until the onset of pupation. In Omphisa, OfSP1 and OfSP2 proteins are produced and released by the larval fat bodies in the fourth and fifth-instar larvae, and the proteins accumulate in the hemolymph until the insects enter diapause. OfSP1 may be reabsorbed by the fat bodies ...Continue Reading

References

Sep 1, 1978·The Journal of Cell Biology·S TojoG R Wyatt
Jan 1, 1991·Annual Review of Entomology·W H Telfer, J G Kunkel
Jan 1, 1994·Archives of Insect Biochemistry and Physiology·J MageeW H Telfer
Jan 1, 1993·Archives of Insect Biochemistry and Physiology·G Jones, N Sarkari
Sep 1, 1996·Insect Biochemistry and Molecular Biology·Norbert H Haunerland
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J K SongH R Kim
Jul 17, 1998·Journal of Molecular Evolution·T BurmesterH Benes
Jul 6, 2000·Insect Biochemistry and Molecular Biology·Tippawan SingtripopSho Sakurai
Dec 1, 2001·Annual Review of Entomology·David L Denlinger
Jul 19, 2002·Archives of Insect Biochemistry and Physiology·Hyang-Mi CheonSook-Jae Seo
Apr 30, 2005·Journal of Insect Science·C K MoreiraO Marinotti

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